Properties of Rikkunshi-to (TJ-43)-induced relaxation of rat gastric fundus smooth muscles
Properties of Rikkunshi-to (TJ-43)-induced relaxation of rat gastric fundus smooth muscles
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DOI：
10.1152/ajpgi.00333.2009
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发表时间：
2010-05-01
影响因子：
4.5
通讯作者：
Suzuki, Hikaru
中科院分区：
文献类型：
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作者：
Kito, Yoshihiko;Suzuki, Hikaru
Kito Y，Suzuki H. Rikkunshi-to（TJ-43）诱导的大鼠胃底平滑肌松弛的性质。美国生理学杂志胃肠和肝脏生理学298：G755-G763，2010。首次发表于2010年2月18日; doi：10.1152/ajpgi.00333.2009。本文研究了胃保护中药力健石汤（TJ-43）对大鼠胃底的舒张作用。使用标准张力和细胞内微电极记录技术进行实验。前列腺素E（2）类似物TJ-43在大鼠胃底环形平滑肌（CSM）条中，在0.5 μ M恩前列地尔（enprostil）引起的收缩过程中，产生剂量依赖性（0.1-5.0 mg/ml）的舒张作用。TJ-43的松弛作用不受河豚毒素或1H [1，2，4]恶二唑并[4，3-a]喹喔啉-1-酮（10 μ M）（可溶性鸟苷酸环化酶的抑制剂）的影响。TJ-43抑制恩前列醇诱导的膜去极化。Apamin（1 μ M）是一种小电导Ca（2+）激活的K（+）（SK）通道的阻断剂，可抑制T-43诱导的膜复极化。TJ-43诱导的舒张是双相的，包括初始的快速舒张，然后是第二次缓慢舒张。apamin可抑制快松弛。应用高K（+）（29.4 mM [K（+）]（o））也消除了TJ-43诱导的快速舒张。在糖尿病Goto-Kakizaki（GK）大鼠胃底CSM条中，TJ-43在恩前列腺素诱导的收缩过程中的舒张反应与对照大鼠条相比增加。这些结果表明TJ-43通过激活SK通道引起的膜超极化引起快速肌松;时间依赖性的慢松弛反映了TJ-43对大鼠胃底CSM的额外指导。由于TJ-43对糖尿病GK大鼠胃底CSM条的舒张作用强于对照大鼠，因此进一步分析该中药可能会导致更好的治疗糖尿病胃轻瘫。
Kito Y, Suzuki H. Properties of Rikkunshi-to (TJ-43)-induced relaxation of rat gastric fundus smooth muscles. Am J Physiol Gastrointest Liver Physiol 298: G755-G763, 2010. First published February 18, 2010; doi:10.1152/ajpgi.00333.2009.-The relaxant effects of Rikkunshi-to (TJ-43), a gastroprotective herbal medicine, on rat gastric fundus were investigated. Experiments were carried out using standard tension and intracellular microelectrode recording techniques. During contraction induced by enprostil (0.5 mu M), a prostaglandin E(2) analog, TJ-43, produced relaxation dose dependently (0.1-5.0 mg/ml) in the rat fundic circular smooth muscle (CSM) strips. The relaxant effects of TJ-43 were not affected by tetrodotoxin or 1 H[1, 2, 4] oxadiazolo [4, 3-a] quinoxalin-1-one (10 mu M), an inhibitor of soluble guanylate cyclase. TJ-43 inhibited enprostil-induced membrane depolarization. Apamin (1 mu M), a blocker of small-conductance Ca(2+)-activated K(+) (SK) channel, inhibited T-43-induced membrane repolarization. TJ-43-induced relaxation was biphasic, comprising of an initial fast followed by a second slow relaxation. The fast relaxation was abolished by apamin. Application of high K(+) (29.4 mM [K(+)](o)) also abolished the fast relaxation induced by TJ-43. In diabetic Goto-Kakizaki (GK) rat fundic CSM strips, the relaxant responses of TJ-43 during enprostil-induced contraction were increased compared with control rat strips. These results indicate that TJ-43 elicited fast muscle relaxation through membrane hyperpolarization induced by the activation of SK channels; the time-dependent slow relaxation reflects an additional direct of TJ-43 on CSM in the rat gastric fundus. Because TJ-43-evoked relaxation of fundic CSM strips was more potent in diabetic GK rat than in control rat, further analysis of this herb could lead to better treatments of diabetic gastroparesis.