Scanning electron microscopy of thyroid cells under fully hydrated conditions--a novel technique for a seasoned procedure: a brief observation.
Scanning electron microscopy of thyroid cells under fully hydrated conditions--a novel technique for a seasoned procedure: a brief observation.
复制标题
完全水合条件下甲状腺细胞的扫描电子显微镜——一种针对成熟程序的新技术：简要观察。
作者：
O. Cohen;R. Beery;S. Levit;J. Ilany;I. Schwartz;M. Shabtai;D. Anaby;David Cohen;R. Alfici;A. Czerniak;A. Karasik
处理甲状腺病变的细针穿刺样品的技术信息WETSEM电子显微镜。湿SEM技术的使用保持了甲状腺细胞的细胞学特征，在大气电子显微镜室中，而不需要固化。图像来自正常和病理甲状腺标本，显示光镜下细胞病理学家无法获得的亚细胞成分。在24件样本中，18件足以进行临床评价。在这18个标本中的16个中，我们可以找到与最终组织学或细胞学诊断（事后）相容的特征。在两种情况下，细胞特征太独特而无法解释。由于该程序相对简单，因此该技术有可能在临床和研究环境中用作光学显微镜的辅助手段。
Technical information for handling fine-needle aspiration samples from thyroid lesions for WETSEM electron microscopy is presented. The use of wet SEM technology maintains cytological features of the thyroid cells, in the atmospheric electronic microscope chamber without the need for solidification. Images are presented from normal and pathological thyroid specimens showing subcellular elements unavailable to the cytopathologist by light microscopy. Of 24 samples, 18 were adequate for clinical evaluation. In 16 of these 18 specimens, we could find features compatible with the final histological or cytological diagnosis (post-hoc). In two cases, the cell features were too unique to be interpretable. Because this procedure is relatively simple, there is potential for the use of this technology as an adjunct to light microscopy in clinical and research settings.
DOI：
10.1056/nejm199302253280807
发表时间：
1993-02
期刊：
The New England journal of medicine
影响因子：
--
作者：
E. Mazzaferri
通讯作者：
E. Mazzaferri