Phenotype and functional identity of GM-CSF-independent dendritic cells generated by long-term propagation of DC progenitor cells in bone marrow cells and skin langerhans cells
Phenotype and functional identity of GM-CSF-independent dendritic cells generated by long-term propagation of DC progenitor cells in bone marrow cells and skin langerhans cells
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DOI：
10.1016/j.transproceed.2005.02.017
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发表时间：
2005-01-01
影响因子：
0.9
通讯作者：
Kimura, H
中科院分区：
文献类型：
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作者：
Katayama, H;Hattori, Y;Kimura, H
有证据表明，通过使用 Flt3L 和白细胞介素 6 (IL-6) 进行长期骨髓细胞 (BMC) 培养，或使用粒细胞巨噬细胞集落刺激因子 (GM-CSF) 和白细胞介素 4 (IL-4) 进行短期 BMC 培养后产生的树突状细胞 (DC) 含有混合 DC 和 M phi 的异质细胞群，无论细胞因子来源如何。通过采用不依赖 GM-CSF 的培养系统，借助 Flt3/Flk-2 配体和 IL-6，以及 BMC 衍生的 DC 和皮肤朗格汉斯细胞 (LC) 的表型表征，揭示了相似的表型。此外，由于标记物的下调，广泛用于大鼠 DC 分离的 CD103 (OX62) 被发现不足以富集 DC。在这方面，最有效的大鼠 DC 选择是通过 C 型凝集素家族成员 CD161a (NKR-P1A) 获得的。尽管与 BMC 衍生的 DC 具有相似的表型，但 LC 的细胞核表现出独特的形态。来自 GM-CSF 受体缺陷小鼠的 Flt3L/IL-6 产生的大量 DC 不表达 NK1.1（NKR-P1B 和 NKR-P1C）。 BMC 衍生的 DC 的特征与皮肤朗格汉斯细胞的特征相同。
Evidence is provided that dendritic cells (DC) generated by either long-term bone marrow cell (BMC) culture with Flt3L and interleukin-6 (IL-6), or after short-term BMC culture with granulocyte macrophage-colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4), contain heterogeneous cell populations of admixed DC and M phi, regardless of the cytokine source. By employing GM-CSF-independent culture systems with the aid of Flt3/Flk-2 ligand and IL-6 and phenotypic characterization of BMC-derived DC and skin Langerhans cells (LC), revealed similar phenotypes. Furthermore, CD103 (OX62), which is widely used for rat DC separation, was found to be insufficient to enrich DC, due to downregulation of the marker. In this regard, the most efficient selection of rat DC, was obtained by CD161a (NKR-P1A), a member of the C-type lectin family. Despite the phenotypic similarity with BMC-derived DC, the nucleus of LC showed a distinct morphology. A large population of DC generated by Flt3L/IL-6 from GM-CSF receptor-deficient mice by do not express NK1.1 (NKR-P1B and NKR-P1C). The profiles for BMC-derived DC were the same as for skin Langerhans cells.