Diversity amongst trigeminal neurons revealed by high throughput single cell sequencing.
Diversity amongst trigeminal neurons revealed by high throughput single cell sequencing.
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DOI：
10.1371/journal.pone.0185543
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Ryba NJP
中科院分区：
文献类型：
--
作者：
Nguyen MQ;Wu Y;Bonilla LS;von Buchholtz LJ;Ryba NJP
三叉神经节含有体感神经元，其检测一系列热、机械和化学线索，并支配头部和颈部的独特感觉区室，包括眼睛、鼻子、嘴、脑膜和触须。我们使用单细胞测序和原位杂交来检查小鼠三叉神经节的细胞多样性，定义了13个神经元簇。我们发现，集群是保守的背根神经节，这表明他们代表不同的功能类的躯体感觉神经元，而不是专业化与他们的感觉目标。值得注意的是，功能上重要的基因（例如机械感觉通道Piezo2和辣椒素门控离子通道Trpv1）分离成多个簇，并且通常在簇内的细胞亚群中表达。因此，13个遗传定义的类别可能是生理上异质的，而不是高度平行的（即，冗余的）感觉输入线。我们的分析利用单细胞测序的力量为计算机表达谱提供了一个独特的平台，补充了其他将基因表达与功能联系起来的方法，并揭示了体感系统中意想不到的多样性。
The trigeminal ganglion contains somatosensory neurons that detect a range of thermal, mechanical and chemical cues and innervate unique sensory compartments in the head and neck including the eyes, nose, mouth, meninges and vibrissae. We used single-cell sequencing and in situ hybridization to examine the cellular diversity of the trigeminal ganglion in mice, defining thirteen clusters of neurons. We show that clusters are well conserved in dorsal root ganglia suggesting they represent distinct functional classes of somatosensory neurons and not specialization associated with their sensory targets. Notably, functionally important genes (e.g. the mechanosensory channel Piezo2 and the capsaicin gated ion channel Trpv1) segregate into multiple clusters and often are expressed in subsets of cells within a cluster. Therefore, the 13 genetically-defined classes are likely to be physiologically heterogeneous rather than highly parallel (i.e., redundant) lines of sensory input. Our analysis harnesses the power of single-cell sequencing to provide a unique platform for in silico expression profiling that complements other approaches linking gene-expression with function and exposes unexpected diversity in the somatosensory system.
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影响因子：
64.5
作者：
Li L;Rutlin M;Abraira VE;Cassidy C;Kus L;Gong S;Jankowski MP;Luo W;Heintz N;Koerber HR;Woodbury CJ;Ginty DD
通讯作者：
Ginty DD
DOI：
10.1016/j.jpain.2014.09.010
发表时间：
2014-12
期刊：
The journal of pain
影响因子：
--
作者：
Goswami SC;Mishra SK;Maric D;Kaszas K;Gonnella GL;Clokie SJ;Kominsky HD;Gross JR;Keller JM;Mannes AJ;Hoon MA;Iadarola MJ
通讯作者：
Iadarola MJ
影响因子：
64.8
作者：
Ranade, Sanjeev S.;Woo, Seung-Hyun;Dubin, Adrienne E.;Moshourab, Rabih A.;Wetzel, Christiane;Petrus, Matt;Mathur, Jayanti;Begay, Valerie;Coste, Bertrand;Mainquist, James;Wilson, A. J.;Francisco, Allain G.;Reddy, Kritika;Qiu, Zhaozhu;Wood, John N.;Lewin, Gary R.;Patapoutian, Ardem
通讯作者：
Patapoutian, Ardem
DOI：
10.1523/jneurosci.5788-12.2013
发表时间：
2013-03-27
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Pogorzala LA;Mishra SK;Hoon MA
通讯作者：
Hoon MA
影响因子：
64.8
作者：
Osteen JD;Herzig V;Gilchrist J;Emrick JJ;Zhang C;Wang X;Castro J;Garcia-Caraballo S;Grundy L;Rychkov GY;Weyer AD;Dekan Z;Undheim EA;Alewood P;Stucky CL;Brierley SM;Basbaum AI;Bosmans F;King GF;Julius D
通讯作者：
Julius D