Patterning and organization of motor neuron dendrites in the Drosophila larva
Patterning and organization of motor neuron dendrites in the Drosophila larva
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DOI：
10.1016/j.ydbio.2009.09.041
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发表时间：
2009-12-15
影响因子：
2.7
通讯作者：
Jan, Yuh-Nung
中科院分区：
文献类型：
--
作者：
Kim, Michael D.;Wen, Yuhui;Jan, Yuh-Nung
运动神经元连接的精确模式取决于树突的正确建立和定位。然而，不同的运动神经元如何定向它们的树突来选择性地建立突触连接还不是很清楚。果蝇神经肌肉系统提供了一个简单的模型来研究基本的组织原则，通过这些原则，不同的运动神经元亚类在中枢神经束内定向它们的树突。在这里，我们使用遗传镶嵌技术来表征果蝇幼虫五个主要神经分支(ISN、ISNb、ISND、SNA和SNC)单个运动神经元的不同树突形态。我们发现，来自不同神经分支的运动神经元将它们的树突投射到神经束背侧区域的大部分刻板印象的内侧外侧区域，提供了对接受区域的完全覆盖。此外，来自不同运动神经元的树突广泛重叠，不分亚类，这表明运动神经元之间的树突-树突相互排斥作用不影响树突场的内侧向外位置。本研究的解剖学数据为不同亚类运动神经元如何组织树突提供了重要信息，并为研究果蝇运动回路中控制突触连接的机制奠定了基础。(C)2009 Elsevier Inc.保留所有权利。
Precise patterns of motor neuron connectivity depend on the proper establishment and positioning of the dendritic arbor. However, how different motor neurons orient their dendrites to selectively establish synaptic connectivity is not well understood. The Drosophila neuromuscular system provides a simple model to investigate the underlying organizational principles by which distinct subclasses of motor neurons orient their dendrites within the central neuropil. Here we used genetic mosaic techniques to characterize the diverse dendritic morphologies of individual motor neurons from five main nerve branches (ISN, ISNb, ISNd, SNa, and SNc) in the Drosophila larva. We found that motor neurons from different nerve branches project their dendrites to largely stereotyped mediolateral domains in the dorsal region of the neuropil providing full coverage of the receptive territory. Furthermore, dendrites from different motor neurons overlap extensively, regardless of subclass, suggesting that repulsive dendrite-dendrite interactions between motor neurons do not influence the mediolateral positioning of dendritic fields. The anatomical data in this study provide important information regarding how different subclasses of motor neurons organize their dendrites and establishes a foundation for the investigation of the mechanisms that control synaptic connectivity in the Drosophila motor circuit. (C) 2009 Elsevier Inc. All rights reserved.