MOLECULAR AND PHYSIOLOGICAL-RESPONSES TO ABSCISIC-ACID AND SALTS IN ROOTS OF SALT-SENSITIVE AND SALT-TOLERANT INDICA RICE VARIETIES
MOLECULAR AND PHYSIOLOGICAL-RESPONSES TO ABSCISIC-ACID AND SALTS IN ROOTS OF SALT-SENSITIVE AND SALT-TOLERANT INDICA RICE VARIETIES
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DOI：
10.1104/pp.107.1.177
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发表时间：
1995-01-01
期刊：
影响因子：
7.4
通讯作者：
Van Der Straeten, D
中科院分区：
文献类型：
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作者：
MOONS, A;BAUW, G;Van Der Straeten, D
以水稻(Oryza sativa L.)Pokkali和Nona Bokra是经典育种中知名的耐盐性供体品种。为了了解它们耐受性的分子基础，人们开始了生理和基因表达的研究。在双向凝胶上分析了脱落酸(ABA)对12d龄的Pokkali、Nona Bokra和盐敏感品种台中N1幼苗根系总蛋白的影响。ABA诱导的蛋白质丰度以耐性最强的Pokkali品种最高。通过部分氨基酸分析，进一步鉴定了耐性和敏感品种根中存在的3种不同程度的ABA响应蛋白。鉴定了一种新的富含组氨酸的蛋白质和两种晚期胚胎发生丰富(LEA)蛋白质。蛋白质免疫印迹分析表明，抗病品种根系中的脱水蛋白和第3组LEA蛋白水平显著高于敏感品种。内源ABA水平在渗透胁迫(150 MM NaC L)胁迫下表现出短暂的升高。与台中N1相比，Nona Bokra的ABA峰值浓度高30倍，Pokkali的ABA峰值浓度高6倍。盐诱导的内源ABA水平和根组织对ABA的较大分子反应均与品种耐性差异有关。
The Indica rice (Oryza sativa L.) varieties Pokkali and Nona Bokra are well-known salt tolerance donors in classical breeding. In an attempt to understand the molecular basis of their tolerance, physiological and gene expression studies were initiated. The effect of abscisic acid (ABA) on total proteins in roots from 12-d-old seedlings of Pokkali, Nona Bokra, and the salt-sensitive cultivar Taichung N1 were analyzed on two-dimensional gels. The abundance of ABA-induced proteins was highest in the most tolerant variety, Pokkali. Three ABA-responsive proteins, present at different levels in roots from tolerant and sensitive varieties, were further characterized by partial amino acid analysis. A novel histidine-rich protein and two types of late embryogenesis abundant (LEA) proteins were identified. Protein immunoblotting revealed that the levels of dehydrins and group 3 LEA proteins were significantly higher in roots from tolerant compared with sensitive varieties. Endogenous ABA levels showed a transient increase in roots exposed to osmotic shock (150 mM NaCl). Peak ABA concentrations were 30-fold higher for Nona Bokra and 6-fold higher for Pokkali compared with Taichung N1. Both the salt-induced endogenous ABA levels and a greater molecular response of root tissue to ABA were associated with the varietal differences in tolerance.