Expression of leukemia/lymphoma related factor (LRF/Pokemon) in human benign prostate hyperplasia and prostate cancer
Expression of leukemia/lymphoma related factor (LRF/Pokemon) in human benign prostate hyperplasia and prostate cancer
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DOI：
10.1016/j.yexmp.2011.01.003
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发表时间：
2011-04-01
影响因子：
3.6
通讯作者：
Agrawal, Devendra K.
中科院分区：
文献类型：
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作者：
Aggarwal, Himanshu;Aggarwal, Anshu;Agrawal, Devendra K.
白血病/淋巴瘤相关因子（LRF），也称为口袋妖怪，是一种属于POK家族的转录抑制因子。它在许多不同的实体瘤中具有致癌作用。在这项研究中，LRF的表达在良性前列腺增生（BPH）和前列腺癌（PC）组织进行了评估。采用RT-PCR、免疫印迹、免疫组织化学和免疫荧光等多种细胞和分子生物学方法研究LRF的功能表达。用石蜡包埋的人BPH和PC组织检测LRF表达。BPH和PC组织切片的组织学染色显示LRF的核表达，在周围基质中具有最小的表达。半定量RT-PCR和Western免疫印迹分析表明，前列腺癌的mRNA转录和蛋白质表达显著高于BPH。LRF的高表达提示其可能在BPH和前列腺癌的发病机制中具有潜在的作用。进一步的研究将有助于阐明LRF在前列腺癌发病机制中可能遵循的机制和信号通路。(C)2011 Elsevier Inc. All rights reserved.
Leukemia/lymphoma related factor (LRF), also known as Pokemon, is a protein that belongs to the POK family of transcriptional repressors. It has an oncogenic role in many different solid tumors. In this study, the expression of LRF was evaluated in benign prostate hyperplastic (BPH) and prostate cancer (PC) tissues. The functional expression of LRF was studied using multiple cellular and molecular methods including RT-PCR, western blotting, immunohistochemistry, and immunofluorescence. Paraffin-embedded human tissues of BPH and PC were used to examine LRF expression. Histological staining of the BPH and PC tissue sections revealed nuclear expression of LRF with minimal expression in the surrounding stroma. The semi-quantitative RT-PCR and western immunoblot analyses demonstrated significantly higher mRNA transcripts and protein expression in PC than BPH. High expression of LRF suggests that it may have a potential role in the pathogenesis of both BPH and prostate cancer. Further studies will help elucidate the mechanisms and signaling pathways that LRF may follow in the pathogenesis of prostate carcinoma. (C) 2011 Elsevier Inc. All rights reserved.