X-ray absorption spectroscopy at a protein crystallography facility: the Canadian Light Source beamline 08B1-1
X-ray absorption spectroscopy at a protein crystallography facility: the Canadian Light Source beamline 08B1-1
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DOI：
10.1107/s090904951204023x
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发表时间：
2012-11-01
影响因子：
2.5
通讯作者：
Fodje, Michel N.
中科院分区：
文献类型：
--
作者：
Cotelesage, Julien J. H.;Grochulski, Pawel;Fodje, Michel N.
现在可以在加拿大光源的加拿大高分子晶体学设施弯曲磁体（CMCF-BM）光束线（08 B1 -1）上对金属蛋白晶体进行X射线吸收光谱（XAS）。最近增加的四元件荧光检测器允许用户通过监测荧光来获取适合于X射线吸收近边结构和扩展的X射线吸收精细结构研究的数据。希望使用XAS补充衍射数据的CMCF光束线用户几乎不需要额外的样品制备。XAS数据收集与用于收集衍射数据的Mx数据收集器软件包集成。用户可以使用主流的XAS数据处理软件包;工作人员也可应要求提供数据处理和解释方面的协助。
It is now possible to perform X-ray absorption spectroscopy (XAS) on metalloprotein crystals at the Canadian Macromolecular Crystallography Facility bend magnet (CMCF-BM) beamline (08B1-1) at the Canadian Light Source. The recent addition of a four-element fluorescence detector allows users to acquire data suitable for X-ray absorption near-edge structure and extended X-ray absorption fine-structure based studies by monitoring fluorescence. CMCF beamline users who wish to supplement their diffraction data with XAS can do so with virtually no additional sample preparation. XAS data collection is integrated with the established Mx Data Collector software package used to collect diffraction data. Mainstream XAS data-processing software packages are available for the users; assistance with data processing and interpretation by staff is also available upon request.