PRESENTATION OF NEUTRALIZING EPITOPES BY ENGINEERED ROTAVIRUS VP7S EXPRESSED BY RECOMBINANT VACCINIA VIRUSES
PRESENTATION OF NEUTRALIZING EPITOPES BY ENGINEERED ROTAVIRUS VP7S EXPRESSED BY RECOMBINANT VACCINIA VIRUSES
复制标题
DOI：
10.1006/viro.1994.1543
复制
发表时间：
1994-10-01
期刊：
影响因子：
3.7
通讯作者：
GREENBERG, HB
中科院分区：
文献类型：
--
作者：
DORMITZER, PR;BOTH, GW;GREENBERG, HB
先前的研究表明，在轮状病毒糖蛋白VP7组装成颗粒的过程中，钙依赖性中和结构域在轮状病毒糖蛋白VP7上形成。在这里，我们证明了表达的重组VP7能够在没有其他轮状病毒蛋白的情况下形成这种中和结构域，但是该结构域是不稳定的。高钙环境、掺入颗粒和中和抗体的结合稳定了表达的VP7上的中和结构域。嵌合的细胞表面锚定分子VP7sc具有增强的与中和抗体反应的能力。这可以解释为什么用表达的天然VP7免疫小鼠具有有限的成功，用VP7sc的白色免疫有效地诱导中和抗体并被动地保护幼仔免于腹泻。与这些结果一致的VP7折叠模型被提出，（C）1994 Academic Press，Inc.
Previous studies showed that a calcium-dependent neutralization domain forms on the rotavirus glycoprotein VP7 during assembly into particles. Here, we demonstrate that expressed, recombinant VP7 is capable of forming this neutralization domain in the absence of other rotavirus proteins, but that the domain is unstable. High calcium environments, incorporation into particles, and binding of neutralizing antibodies stabilize the neutralization domain on expressed VP7. A chimeric, cell surface-anchored molecule, VP7sc, has an enhanced ability to react with neutralizing antibodies. This may explain why immunization of mice with expressed native VP7 has had limited success white immunization with VP7sc efficiently induced neutralizing antibodies and passively protected pups from diarrhea. A model of VP7 folding consistent with these results is presented, (C) 1994 Academic Press, Inc.