MIR205HG facilitates carcinogenesis of lung squamous cell carcinoma in vitro revealed by long noncoding RNA profiling
MIR205HG facilitates carcinogenesis of lung squamous cell carcinoma in vitro revealed by long noncoding RNA profiling
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DOI：
10.1093/abbs/gmaa006
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发表时间：
2020-04-01
影响因子：
3.7
通讯作者：
Chen, Liang'an
中科院分区：
文献类型：
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作者：
Chang, Yan;Xue, Xinying;Chen, Liang'an
作为非小细胞肺癌的一种亚型，肺鳞状细胞癌（LUSC）占所有肺癌的五分之一。不幸的是，尚未确定具体的靶向畸变。因此，确定LUSC中异常调控的基因具有巨大的紧迫性和潜力。在此，对五对LUSC样品及其相应的邻近组织进行全转录组测序。我们的结果显示，在所有五个LUSC样品中，CTD-2562J17.6和FENDRR显著下调，而MIR 205 HG、LNC_000378、RP 11 -116G8.5、RP 3 -523K23.2和RP 5 -968D22.1显著上调。重要的是，MIR 205 HG在LUSC临床样品以及LUSC细胞系中上调。有趣的是，我们的结果表明MIR 205 HG的表达水平与恶性程度呈正相关。此外，MIR 205 HG是LUSC细胞生长和细胞迁移所必需的。最重要的是，我们的结果表明MIR 205 HG通过调节Bcl-2和Bax抑制LUSC凋亡。总之，我们的数据揭示了控制LUSC致癌的lncRNA调控关系，并为LUSC提供了潜在的新型诊断标志物和治疗靶点。
As a subtype of non-small-cell lung cancer, lung squamous cell carcinoma (LUSC) accounts for one-fifth of all lung cancers. Unfortunately, no specific targetable aberration has yet been identified. Hence, it is of huge urgency and potential to identify aberrantly regulated genes in LUSC. Here, five pairs of LUSC samples and their corresponding adjacent tissues were subject to whole transcriptome sequencing. Our results showed that CTD-2562J17.6 and FENDRR were significantly downregulated while MIR205HG, LNC_000378, RP11-116G8.5, RP3-523K23.2, and RP5-968D22.1 were significantly upregulated in all five LUSC samples. Importantly, MIR205HG was upregulated in LUSC clinical samples as well as in LUSC cell lines. Interestingly, our results demonstrated that the expression level of MIR205HG is positively correlated with the malignancy. In addition, MIR205HG is required for LUSC cell growth and cell migration. Most importantly, our results showed that MIR205HG prohibits LUSC apoptosis via regulating Bcl-2 and Bax. Taken together, our data shed lights on the lncRNA regulatory nexus that controls the carcinogenesis of LUSC and provided potential novel diagnostic markers and therapeutic targets for LUSC.