Fractionation and characterization of Tetrahymena histone in comparison with mammalian histones.
Fractionation and characterization of Tetrahymena histone in comparison with mammalian histones.
复制标题
四膜虫组蛋白的分离和表征与哺乳动物组蛋白的比较。
DOI：
10.1093/oxfordjournals.jbchem.a129563
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发表时间：
1971
影响因子：
2.7
通讯作者：
K. Iwai
中科院分区：
文献类型：
--
作者：
K. Hamana;K. Iwai
从对数生长期梨形四膜虫无微核GL株细胞核制备的染色质中提取全组蛋白和组蛋白组分。它们在CM-纤维素柱上的色谱行为（使用我们的方法），也在Amberlite CG-50和Bio-Gel P-60上的色谱行为与大鼠肝脏，大鼠胸腺和小牛胸腺的组蛋白进行了比较。将通过Amberlite或Bio-Gel层析获得的原生动物组蛋白组分在CM-纤维素上再层析，并分离出8个或9个组分。这些组分中的大多数在聚丙烯酰胺凝胶电泳上给出具有不同迁移率的单一条带，但它们可以被分为五种或六种主要类型的分子种类，其具有不同的氨基酸组成和分子大小，对应于五种主要类型的哺乳动物组蛋白。相应的原生动物和哺乳动物类型之间的相似性和差异表明，每个序列的保守性和变异性都必须在从共同祖先进化到原生动物和哺乳动物的过程中发生。
Whole histone and histone fractions were extracted from chromatin prepared from the nuclei of an amicronucleate strain GL of Tetrahymena pyriformis during exponential growth. Their chromatographic behaviors on a CM-cellulose column (using our method) and also on Amberlite CG-50 and Bio-Gel P-60 were compared with those of histones of rat liver, rat thymus, and calf thymus. The protozoan histone fractions obtained by Amberlite or Bio-Gel chromatography were rechromatographed on CM-cellulose and eight or nine fractions were separated. Most of these fractions gave single bands with different mobilities on polyacrylamide gel electrophoresis, but they could be grouped into five or six main types of molecular species with different amino acid compositions and molecular sizes corresponding to the five main types of mammalian histones. Similarities and differences between the corresponding protozoan and mammalian types suggest that both conservation and variation of each sequence must have occurred during evolution from a common ancestor to protozoa and to mammals.