Glutathione and a UV light-induced glutathione S-transferase are involved in signaling to chalcone synthase in cell cultures
Glutathione and a UV light-induced glutathione S-transferase are involved in signaling to chalcone synthase in cell cultures
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DOI：
10.1105/tpc.12.10.1939
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发表时间：
2000-10-01
期刊：
影响因子：
11.6
通讯作者：
Frohnmeyer, H
中科院分区：
文献类型：
--
作者：
Loyall, L;Uchida, K;Frohnmeyer, H
紫外光照射可以刺激关键酶查尔酮合成酶(CHS)基因的表达，从而导致欧芹细胞培养物中保护性类黄酮的产生。CHS转录在3到4小时后增加，早期基因参与CHS启动子的信号转导。通过大规模筛选中的荧光差异显示技术，分离了几个早期紫外光诱导的基因。其中，一种新的谷胱甘肽S转移酶(PcGST1)在2小时内被诱导，并先于CHS表达。在含有CHS启动子/荧光素酶报告基因(CHS-Luc)的转化细胞系中过表达PcGST1影响了Luc转录的启动。在暗培养的细胞中，补充谷胱甘肽立即在2小时内刺激CHS-LOC的表达，并在紫外线照射的细胞中导致双相诱导。我们的数据表明谷胱甘肽和PcGST1参与了紫外光依赖的CHS信号转导途径的早期事件。在这种情况下，细胞的氧化状态作为一个中央调节元件。
UV irradiation stimulates expression of the gene encoding the key enzyme chalcone synthase (CHS), which leads to the generation of protective flavonoids in parsley cell cultures. CHS transcripts increase after 3 to 4 hr, and early genes are involved in the signal transduction to the CHS promoter. By using the fluorescent differential display technique in a large-scale screening, several early UV light-induced genes were isolated. Of these, a novel glutathione S-transferase (PcGST1) is induced within 2 hr and precedes CHS expression. Overexpression of PcGST1 in transformed cell lines containing a CHS promoter/luciferase reporter (CHS-LUC) affected the onset of LUC transcription. Supplementing these cell lines with glutathione immediately stimulated CHS-LOC expression within 2 hr in dark-incubated cells and resulted in a biphasic induction profile in UV-irradiated cells. Our data indicate the involvement of glutathione and PcGST1 in early events of a UV light-dependent signal transduction pathway to CHS. In this context, the oxidative status of a cell acts as a central regulating element.