DHA SYSTEM MEDIATING AEROBIC AND ANAEROBIC DISSIMILATION OF GLYCEROL IN KLEBSIELLA-PNEUMONIAE NCIB-418
DHA SYSTEM MEDIATING AEROBIC AND ANAEROBIC DISSIMILATION OF GLYCEROL IN KLEBSIELLA-PNEUMONIAE NCIB-418
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DOI：
10.1128/jb.151.2.591-599.1982
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发表时间：
1982-01-01
影响因子：
3.2
通讯作者：
LIN, ECC
中科院分区：
文献类型：
--
作者：
FORAGE, RG;LIN, ECC
在K. pneumoniae NCIB 418中，通常负责在甘油和sn-甘油3-磷酸（glp系统）上需氧生长的途径被超抑制。甘油上的需氧生长可以通过NAD连接的甘油脱氢酶和dha系统的ATP依赖性二羟基丙酮激酶的干预而发生，该激酶通常仅由甘油或二羟基丙酮厌氧诱导。缺乏二羟丙酮激酶的Tn 5插入突变体提供了dha系统负责甘油有氧和无氧异化的确凿证据。设计了对该酶特异性的酶偶联测定。glp系统的自发重新激活是通过选择在3-磷酸锡-甘油或限制甘油作为唯一碳和能量来源的有氧生长来实现的。这一制度的表现形式成为构成性的。glp系统的需氧操作通过分解代谢物抑制而高度抑制dha系统酶的合成。
In K. pneumoniae NCIB 418, the pathways normally responsible for aerobic growth on glycerol and sn-glycerol 3-phosphate (the glp system) are superrepressed. Aerobic growth on glycerol can take place by the intervention of the NAD-linked glycerol dehydrogenase and the ATP-dependent dihydroxyacetone kinase of the dha system normally inducible only anaerobically by glycerol or dihydroxyacetone. Conclusive evidence that the dha system is responsible for aerobic and anaerobic dissimilation of glycerol was provided by a Tn5 insertion mutant lacking dihydroxyacetone kinase. An enzymatically coupled assay specific for this enzyme was devised. Spontaneous reactivation of the glp system was achieved by selection for aerobic growth on sn-glycerol 3-phosphate or on limiting glycerol as the sole C and energy source. The expression of this system became constitutive. Aerobic operation of the glp system highly represses synthesis of the dha system enzymes by catabolite repression.