Growth rates and tolerance to low water temperatures of freshwater bacterioplankton strains: ecological insights from shallow hypereutrophic lakes in Japan
Growth rates and tolerance to low water temperatures of freshwater bacterioplankton strains: ecological insights from shallow hypereutrophic lakes in Japan
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淡水浮游细菌菌株的生长速度和对低水温的耐受性：来自日本浅层富营养化湖泊的生态见解
DOI：
10.1007/s10750-016-3045-7
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Seiji Hayashi
中科院分区：
文献类型：
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作者：
Keiji Watanabe;Yuichi Ishii;Nobuyuki Komatsu;Tatsumi Kitamura;Mirai Watanabe;Shigeki Yamamura;Akio Imai;Seiji Hayashi
为了研究夏季和冬季可培养的淡水浮游细菌的差异，我们在两个冬季对日本两个富营养化的温带湖泊的低温表层水中的特定浮游细菌进行了检测，并将我们的结果与以前发表的数据进行了比较。我们使用了大小排除分析方法(SEAM)，这是一种基于培养的方法，可以简单而有效地分离典型的淡水浮游细菌。在冬季样本中检测到的特定集群是红假单胞菌。BAL47星系团(利马和利马)，一个主要的和普遍存在的淡水世系，以及LiUU-5-340星系团。为了确定冬季特有群体对低水温的耐受性，我们还比较了几个属于红假单胞菌的典型淡水浮游细菌群在5℃下的生长速度。BAL47、必需性多核杆菌、GKS98、LiUU-5-340、IRD18C08；放线菌露娜-1、露娜-2。在冬季样品中，SEAM对淡水浮游细菌集群/亚集群的特定可探测性与每个分离物的低温特定生长特征有很大关系。对水温的响应是淡水浮游细菌群落组成的关键控制因素。这些结果为温带气候带中几个普遍存在的可培养淡水浮游细菌群对水温的特殊反应提供了重要的见解。
To investigate differences in culturable freshwater bacterioplankton between summer and winter, we examined specific bacterioplankton in the low temperature surface water of two shallow, hypereutrophic, temperate lakes in Japan over two winters, and compared our results with the previously published data. We used the size-exclusion assay method (SEAM), a cultivation-based approach that simply and effectively isolates typical freshwater bacterioplankton. The specific clusters detected in the winter samples were theRhodoferaxsp. BAL47 cluster (LimA and LimC), a predominant and ubiquitous freshwater lineage, and the LiUU-5-340 cluster. To confirm tolerance to low water temperature of winter-specific groups, we also compared growth rates at 5°C among several pure strains of typical freshwater bacterioplankton clusters belonging to theRhodoferaxsp. BAL47,Polynucleobacter necessarius, GKS98, LiUU-5-340, and IRD18C08 of the classBetaproteobacteria, and the Luna-1 and Luna-2 of the classActinobacteria. Specific detectability of freshwater bacterioplankton clusters/subclusters by SEAM in the winter sample substantially correlated with the low temperature-specific growth characteristics of each isolate. Response to water temperature is a key control factor in freshwater bacterioplankton assemblage composition. These results provide important insights into the specific response to water temperature of several ubiquitous culturable freshwater bacterioplankton clusters inhabiting a temperate climate zone.