Intragraft expression of IL-10 messenger RNA: a novel correlate of renal allograft rejection.
Intragraft expression of IL-10 messenger RNA: a novel correlate of renal allograft rejection.
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IL-10 信使 RNA 的移植物内表达：肾同种异体移植排斥的新相关因素。
DOI：
10.1038/ki.1995.440
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发表时间：
1995
影响因子：
19.6
通讯作者：
Stenzel,KH
中科院分区：
文献类型：
--
作者：
Xu,GP;Sharma,VK;Li,B;Bologa,R;Li,Y;Mouradian,J;Wang,J;Serur,D;Rao,V;Stenzel,KH
移植物内IL-10信使RNA表达：肾移植排斥反应的新相关因素。一个主要的概念性进展是I型细胞因子（如IL-2和IFN-γ）增强细胞免疫并具有宿主保护性，而II型细胞因子（如IL-4和IL-10）抑制细胞免疫并促进感染进展。我们探讨了在人肾移植排斥反应中移植物内I型和II型细胞因子的表达。从98例同种异体移植物活检组织中分离RNA，并使用逆转录-PCR扩增和鉴定移植物内编码IL-2、IFN-γ、IL-4或IL-10的mRNA表达。同时观察移植物内IL-7 mRNA和TGF-β1 mRNA的表达。我们的研究表明：（a）移植物内IL-10 mRNA和IL-2 mRNA的表达与急性排斥反应显著相关;（B）IL-4、IL-7、IFN-γ和TGF-β1 mRNA的表达与急性排斥反应无关;（c）IL-10不能阻止IFN-γ、IL-2、IL-7或TGF-β 1的体内表达。我们的研究首次确定了移植物内IL-10 mRNA表达与急性排斥反应之间的显著相关性，并表明能够抑制IL-10表达的治疗策略可能在预防急性排斥反应中具有价值。
Intragraft expression of IL-10 messenger RNA: A novel correlate of renal allograft rejection. A major conceptual advance is the formulation that type I cytokines (such as IL-2 and IFN-γ) enhance cellular immunity and are host-protective, and that type II cytokines (such as IL-4 and IL-10) dampen cellular immunity and facilitate the progression of infection. We have explored the intragraft expression of type I and type II cytokines during human renal allograft rejection. RNA was isolated from 98 allograft biopsies, and reverse transcription-PCR was used to amplify and identify intragraft expression of mRNA encoding IL-2, IFN-γ, IL-4, or IL-10. Intragraft expression of IL-7 mRNA and TGF-β1 mRNA was also investigated. Our investigation demonstrated that: (a) intragraft expression of IL-10 mRNA and IL-2 mRNA are significant correlates of acute rejection; (b) IL-4, IL-7, IFN-γ and TGF-β1 mRNA expression do not correlate with acute rejection; and (c) IL-10 does not preventin vivoexpression of IFN-γ, IL-2, IL-7, or TGF-βl. Our studies identify, for the first time, a significant association between intragraft IL-10 mRNA expression and acute rejection, and suggest that treatment strategies capable of constraining IL-10 expression might be of value in the prevention of acute rejection.