The microsomal metabolism of benzo(a) pyrene phenols.
The microsomal metabolism of benzo(a) pyrene phenols.
复制标题
苯并（a）芘酚的微粒体代谢。
DOI：
10.1016/0006-291x(78)90905-1
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发表时间：
1978
影响因子：
3.1
通讯作者：
R. Prough
中科院分区：
文献类型：
--
作者：
J. Capdevila;R. Estabrook;R. Prough
含有大鼠肝微粒体、3-或9-羟基苯并（a）芘、氧和NADPH的孵育混合物的重复扫描差谱分析显示，形成了在400-450 nm区域具有吸光度的产物。根据色谱保留时间、吸光度和荧光光谱，9-羟基苯并（a）芘代谢的两种主要产物可能是二酚。在3-羟基苯并芘的稳态代谢过程中存在类似于酚类而不是醌类的光谱中间体，这强烈表明主要产物是缓慢氧化产生3，6-醌的二酚和/或肝微粒体中存在活性醌还原酶。
Analysis of repetitive scan difference spectra of incubation mixtures containing rat liver microsomes, 3- or 9-hydroxybenzo(a)pyrene, oxygen, and NADPH shows the formation of products with absorbance in the 400–450 nm region. Based on the chromatographic retention time, absorbance, and fluorescence spectra, the two major products of 9-hydroxybenzo(a)pyrene metabolism may be diphenols. The existence of spectral intermediates which resemble phenols rather than quinones during the steady-state metabolism of 3-hydroxybenzo(a)pyrene strongly indicates that either the major product is a diphenol which slowly oxidizes to yield 3,6-quinone and/or that an active quinone reductase exists in liver microsomes.