The effect of enzymatically degradable poly(ethylene glycol) hydrogels on smooth muscle cell phenotype
The effect of enzymatically degradable poly(ethylene glycol) hydrogels on smooth muscle cell phenotype
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DOI：
10.1016/j.biomaterials.2007.09.036
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发表时间：
2008-01-01
期刊：
影响因子：
14
通讯作者：
Frey, Peter
中科院分区：
文献类型：
--
作者：
Adeloew, Catharina;Segura, Tatiana;Frey, Peter
由于平滑肌细胞（SMC）的去分化而形成的瘢痕组织是工程化膀胱组织时面临的主要问题之一。此外，用于再生SMC层的细胞来源也是有限的。在这里，我们探索在酶可降解的聚乙二醇（PEG）水凝胶支架中培养的人间充质干细胞（MCS）是否可以分化为SMC样细胞。我们探索了当在这些支架内培养原代人SMC时，可以实现较少合成的SMC表型的程度。观察到当MSC和SMC都在PEG水凝胶支架中培养，而不是在传统的组织培养塑料上培养时，它们上调与较少合成的SMC表型相关的标志物，降低015整联蛋白和THY-1的表达，以及α-平滑肌肌动蛋白（α SMA）和肌球蛋白的表达增加。此外，我们表明，在PEG水凝胶中培养的MSC和SMC能够在培养中增殖并表达基质金属蛋白酶长达21天，即研究的持续时间。这项研究解决了细胞命运的细胞微环境的重要性，并提出了合成的指导性生物材料作为一种手段，直接细胞分化和规避膀胱重建过程中的瘢痕组织形成。(C)2007爱思唯尔有限公司保留所有权利。
The formation of scar tissue due to dedifferentiation of smooth muscle cells (SMCs) is one of the major issues faced when engineering bladder tissue. Furthermore, cell sources for regenerating the SMC layer are also limiting. Here we explore if human mesenchymal stem cells (MCSs), cultured in enzymatically degradable poly(ethylene glycol) (PEG) hydrogel scaffolds can be differentiated into SMC-like cells. We explored the degree to which a less synthetic SMC phenotype can be achieved when primary human SMCs are cultured within these scaffolds, It was observed that when both MSCs and SMCs are cultured in the PEG hydrogel scaffolds, but not on traditional tissue culture plastic, they up-regulate markers associated with the less synthetic SMC phenotype, decreased expression of 015 integrin and THY-1, and increased expression of alpha-smooth muscle actin (alpha SMA) and myosin. Furthermore, we show that MSCs and SMCs cultured in the PEG hydrogels are able to proliferate and express matrix metalloproteinases for up to 21 d in culture, the duration of the study. This study addresses the importance of the cellular microenvironment on cell fate, and proposes synthetic instructive biomaterials as a means to direct cell differentiation and circumvent scar tissue formation during bladder reconstruction. (C) 2007 Elsevier Ltd. All rights reserved.