Analysis of genetic diversity in the Brassica napus L. gene pool using SSR markers
Analysis of genetic diversity in the Brassica napus L. gene pool using SSR markers
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DOI：
10.1007/s10722-004-5541-2
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发表时间：
2006-06-01
影响因子：
2
通讯作者：
Snowdon, RJ
中科院分区：
文献类型：
--
作者：
Hasan, M;Seyis, F;Snowdon, RJ
甘蓝型油菜(Brassica napus SSP.通过获得广泛的冬、春油菜、饲料和叶用油菜基因库材料的SSR基因座上的详细分子遗传信息，对甘蓝型油菜(Napus)初级基因库进行了研究。调查的植物材料选自从欧洲基因库材料发展而来的甘蓝型油菜初步核心种质，目的是尽可能广泛地涵盖该物种中存在的多样性，不包括瑞典人(B.napus SSP.油菜Napobrassica(L.)Hanelt)。利用分布在甘蓝型油菜基因组上的可公开获得的作图SSR标记，对一组96个基因类型进行了表征。来自30个SSR引物组合的等位基因信息在51个多态座位上扩增出220个等位基因，为所有基因型提供了独特的遗传指纹图谱。UPGMA聚类法能够识别出四个遗传多样性不断增加的群体：(1)春季油料和饲料；(2)冬季油料；(3)冬季饲料；(4)蔬菜基因型。英国春甘蓝和日本春甘蓝的等位基因变异最大，韩国和日本的两个冬油菜品种的等位基因变异最大。出人意料的是，排在第二位的最明显的基因类型是两个古老的冬季油籽品种，分别来自德国和乌克兰。一些其他材料也被发现在遗传上与其他相同类型的材料不同。所获得的分子遗传信息能够为油菜育种鉴定未利用的遗传变异，并在增加油菜杂交种的杂种优势方面具有潜在的意义。
Genetic diversity throughout the rapeseed (Brassica napus ssp. napus) primary gene pool was examined by obtaining detailed molecular genetic information at simple sequence repeat (SSR) loci for a broad range of winter and spring oilseed, fodder and leaf rape gene bank accessions. The plant material investigated was selected from a preliminary B. napus core collection developed from European gene bank material, and was intended to cover as broadly as possible the diversity present in the species, excluding swedes (B. napus ssp. napobrassica (L.) Hanelt). A set of 96 genotypes was characterised using publicly available mapped SSR markers spread over the B. napus genome. Allelic information from 30 SSR primer combinations amplifying 220 alleles at 51 polymorphic loci provided unique genetic fingerprints for all genotypes. UPGMA clustering enabled identification of four general groups with increasing genetic diversity as follows (1) spring oilseed and fodder; (2) winter oilseed; (3) winter fodder; (4) vegetable genotypes. The most extreme allelic variation was observed in a spring kale from the United Kingdom and a Japanese spring vegetable genotype, and two winter rape accessions from Korea and Japan, respectively. Unexpectedly the next most distinct genotypes were two old winter oilseed varieties from Germany and Ukraine, respectively. A number of other accessions were also found to be genetically distinct from the other material of the same type. The molecular genetic information gained enables the identification of untapped genetic variability for rapeseed breeding and is potentially interesting with respect to increasing heterosis in oilseed rape hybrids.