Molecular weight of human high-molecular-weight kininogen light chain by equilibrium sedimentation in an air-driven ultracentrifuge.
Molecular weight of human high-molecular-weight kininogen light chain by equilibrium sedimentation in an air-driven ultracentrifuge.
复制标题
通过在空气驱动超速离心机中平衡沉降测定人高分子量激肽原轻链的分子量。
DOI：
10.1016/0003-2697(83)90747-9
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发表时间：
1983
影响因子：
2.9
通讯作者：
Halvorson,HR
中科院分区：
文献类型：
--
作者：
Bock,PE;Halvorson,HR
高分子量激肽原是体内凝血系统的一种非酶糖蛋白，被激肽释放酶降解为该系统激活的早期事件。被切割的激肽原轻链保留了与该系统的其他成员前激肽释放酶和因子XI形成特定的非共价复合体的能力。我们用气动台式超速离心机在不同密度的缓冲液中平衡沉淀法测定了人激肽原轻链的分子量。所得的相对分子质量(30,500±800g/m ol)和部分比体积(0.660±0.008 m l/g)与高分子量激肽原碳水化合物中相当大一部分与轻链有关的观点一致。这样的精确度相对容易达到。详细介绍了这些步骤，以及误差传播的表达式，以便于该技术的应用。
High-molecular-weight kininogen, a nonenzymatic glycoprotein of the intrinsic blood coagulation system, is proteolytically cleaved by kallikrein as an early event in the activation of this system. The light chain of cleaved kininogen retains the ability to form specific noncovalent complexes with prekallikrein and factor XI, other members of this system. We have determined the molecular weight of human kininogen light chain by equilibrium sedimentation in buffers of differing density, using an air-driven benchtop ultracentrifuge. The resulting molecular weight (30,500±800 g/mol) and partial specific volume (0.660 ± 0.008 ml/g) are consistent with the idea that a sizeable fraction of the carbohydrate of high-molecular-weight kininogen is associated with the light chain. This level of precision is relatively easy to attain. The procedures are detailed, along with expressions for error propagation, to permit ready application of the technique.