Ozone exposure, antioxidant genes, and lung function in an elderly cohort: VA normative aging study
Ozone exposure, antioxidant genes, and lung function in an elderly cohort: VA normative aging study
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DOI：
10.1136/oem.2007.035253
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发表时间：
2008-11-01
影响因子：
4.9
通讯作者：
Schwartz, J.
中科院分区：
文献类型：
--
作者：
Alexeeff, S. E.;Litonjua, A. A.;Schwartz, J.
背景：臭氧（O-3）暴露已知会引起氧化应激。这项研究调查了O-3对老年人肺功能的急性影响，这是一个可疑的风险群体。然后调查是否抗氧化基因的遗传多态性（血红素氧合酶-1（HMOX 1）和谷胱甘肽S-转移酶pi（GSTP 1））修改这些association.Methods：1100老年男性从规范的老化研究进行了检查，其肺功能（用力肺活量（FVC）和用力呼气量在1秒（FEV 1）），每3年从1995年至2005年测量。该研究对GSTP 1 Ile 105 Val和Ala 114 Val多态性以及HMOX 1启动子中的（GT）n重复多态性进行了基因分型，将重复序列分类为短重复序列（n= 25）。在大波士顿地区的各个地点连续测量环境O-3。混合线性模型，调整已知confersider.Results：在过去的48小时内增加15 ppb的O-3与FEV 1下降1.25%（95%CI：-1.96%至0.54%）。HMOX 1中存在长（GT）n重复（-1.38%，95% CI：-2.11%至-0.65%）或GST P1中存在编码Val 105的等位基因（-1.69%，95% CI：-2.63%至-0.75%）时，该估计效应恶化。O-3对FEV 1的影响在携带GSTP 1 105 Val变异体和HMOX 1长（GT）n重复序列的受试者中更强（-1.94%，95%CI：-2.89%至-0.98%）。FVC和O-3 exposure.Conclusions之间也发现了类似的关联：我们的研究结果表明，O-3对老年人的肺功能有急性影响，抗氧化基因中存在特定的多态性可能会改变这种影响。
Background: Ozone (O-3) exposure is known to cause oxidative stress. This study investigated the acute effects of O-3 on lung function in the elderly, a suspected risk group. It then investigated whether genetic polymorphisms of antioxidant genes (heme oxygenase-1 (HMOX1) and glutathione S-transferase pi (GSTP1)) modified these associations.Methods: 1100 elderly men from the Normative Aging Study were examined whose lung function (forced vital capacity (FVC) and forced expiratory volume in 1 second (FEV1)) was measured every 3 years from 1995 to 2005. The study genotyped the GSTP1 Ile105Val and Ala114Val polymorphisms and the (GT) n repeat polymorphism in the HMOX1 promoter, classifying repeats as short (n= 25). Ambient O-3 was measured continuously at locations in the Greater Boston area. Mixed linear models were used, adjusting for known confounders.Results: A 15 ppb increase in O-3 during the previous 48 h was associated with a 1.25% decrease in FEV1 (95% CI: -1.96% to -0.54%). This estimated effect was worsened with either the presence of a long (GT) n repeat in HMOX1 (-1.38%, 95% CI: -2.11% to -0.65%) or the presence of an allele coding for Val105 in GSTP1 (-1.69%, 95% CI: -2.63% to -0.75%). A stronger estimated effect of O-3 on FEV1 was found in subjects carrying both the GSTP1 105Val variant and the HMOX1 long (GT) n repeat (-1.94%, 95% CI: -2.89% to -0.98%). Similar associations were also found between FVC and O-3 exposure.Conclusions: Our results suggest that O-3 has an acute effect on lung function in the elderly, and the effects may be modified by the presence of specific polymorphisms in antioxidant genes.