Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells.
Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells.
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DOI：
10.1091/mbc.e18-05-0282
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发表时间：
2018-08-15
影响因子：
3.3
通讯作者：
Walther TC
中科院分区：
文献类型：
--
作者：
Jayson CBK;Arlt H;Fischer AW;Lai ZW;Farese RV Jr;Walther TC
Rab GTP酶募集外周膜蛋白，并可以定义细胞器的身份。Rab18定位于内质网（ER），但也定位于脂滴（LD），在那里它参与效应蛋白的募集和定义LD身份。在这里，我们研究了Rab18在人乳腺癌细胞系中的定位和功能。Rab18定位于ER和LD膜上LD诱导，后者取决于Rab18激活状态。在缺乏Rab18的细胞中，LD的大小和数量适度减少，但我们几乎没有发现Rab18在LD形成，细胞饥饿时LD周转或几种蛋白质靶向LD中的功能的证据。我们的结论是，Rab18是不是一个一般的，必要的组成部分的蛋白质机械参与LD生物合成或营业额。
Rab GTPases recruit peripheral membrane proteins and can define organelle identity. Rab18 localizes to the endoplasmic reticulum (ER) but also to lipid droplets (LDs), where it has been implicated in effector protein recruitment and in defining LD identity. Here, we studied Rab18 localization and function in a human mammary carcinoma cell line. Rab18 localized to the ER and to LD membranes on LD induction, with the latter depending on the Rab18 activation state. In cells lacking Rab18, LDs were modestly reduced in size and numbers, but we found little evidence for Rab18 function in LD formation, LD turnover on cell starvation, or the targeting of several proteins to LDs. We conclude that Rab18 is not a general, necessary component of the protein machinery involved in LD biogenesis or turnover.