20 (s)-ginsenoside Rg3-loaded magnetic human serum albumin nanospheres applied to HeLa cervical cancer cells in vitro
20 (s)-ginsenoside Rg3-loaded magnetic human serum albumin nanospheres applied to HeLa cervical cancer cells in vitro
复制标题
20(s)-人参皂苷Rg3磁性人血清白蛋白纳米球体外作用于HeLa宫颈癌细胞
DOI：
10.3233/bme-141008
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发表时间：
2014-01-01
影响因子：
1
通讯作者：
Tang, Qiusha
中科院分区：
文献类型：
--
作者：
Yang, Rui;Chen, Daozhen;Tang, Qiusha
20（s）-Rg 3是从中药红参中提取的。然而，由于其水溶性差和口服生物利用度低，20（s）-Rg 3的使用受到限制。本研究旨在探索一种制备20（s）-Rg 3磁性人血清白蛋白纳米球（20（s）-Rg 3/HSAMNP）的方法，通过改变剂型来提高其水溶性和生物利用度。采用去溶剂交联法制备了20（s）-Rg 3/HSAMNP。检测20（s）-Rg 3/HSAMNP的特性。MTT法检测20（s）-Rg 3/HSAMNP对人宫颈癌细胞的增殖抑制作用，流式细胞仪检测20（s）-Rg 3/HSAMNP对人宫颈癌细胞的凋亡率。TEM分析表明20（s）-Rg 3/HSAMNP为近似球形，大小均匀。热力学测试表明，特定浓度的磁流体在42-65 ℃之间稳定升温。铁含量约为3 mg/mL。药物包封率约为70%。20（s）-Rg 3/HSAMNP联合磁热疗法抑制细胞生长和诱导细胞凋亡的潜力明显高于其他组。制备了一种新的20（s）-Rg 3剂型，该剂型与热疗联合应用能有效诱导体外培养的HeLa宫颈癌细胞凋亡。
20(s)-ginsenoside Rg3 is extracted from traditional Chinese medicine, red ginseng. However, due to its poor aqueous solubility and low oral bioavailability, the use of 20(s)-Rg3 is limited. This study aimed to explore a method of preparing nano-sized 20(s)-ginsenoside Rg3 particle named 20(s)-ginsenoside Rg3-loaded magnetic human serum albumin nanospheres (20(s)-Rg3/HSAMNP) to change dosage form to improve its aqueous solubility and bioavailability. 20(s)-Rg3/HSAMNP were prepared by the desolvation-crosslinking method. The character of 20(s)-Rg3/HSAMNP was detected. An anti-proliferative effect and cell apoptosis rates of 20(s)-Rg3/HSAMNP on human cervical cancer cells were determined by the MTT assay and flow cytometry, respectively. TEM analysis showed that 20(s)-Rg3/HSAMNP were approximately spherical and uniform in size. Thermodynamic testing showed that the corresponding magnetic fluid of a specific concentration rosed to a steady temperature of 42-65 degrees C. Iron content was approximately 3 mg/mL. Drug encapsulation efficiency was approximately 70%. The potential of 20(s)-Rg3/HSAMNP combined with magnetic hyperthermia therapy to inhibit cell growth and induce apoptosis was much more prominent than that of the other groups. A new dosage form of 20(s)-Rg3 was prepared, which effectively induced apoptosis in HeLa cervical cancer cells in vitro when combined with hyperthermia.