Transsynaptic tracing with vesicular stomatitis virus reveals novel retinal circuitry.
Transsynaptic tracing with vesicular stomatitis virus reveals novel retinal circuitry.
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DOI：
10.1523/jneurosci.0245-12.2013
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发表时间：
2013-01-02
期刊：
影响因子：
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通讯作者：
Cepko CL
中科院分区：
文献类型：
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作者：
Beier KT;Borghuis BG;El-Danaf RN;Huberman AD;Demb JB;Cepko CL
嗜神经病毒作为跨突触示踪剂的使用最早出现在20世纪60年代，但直到最近，这种病毒才作为一种标记神经回路的方法而受到欢迎。逆行单突触追踪向量的发展使突触前源可视化到突触后神经元的定义集。在这里，我们描述了一种基于水疱性口炎病毒（VSV）的新型病毒示踪剂的首次应用，该示踪剂指导病毒在特定细胞类型之间的逆行跨突触传播。我们在小鼠视网膜中使用这种病毒来显示星爆无突细胞（SACs）与其已知的突触伙伴，方向选择性视网膜神经节细胞（DSGCs）之间的连接，以及发现SACs与其他视网膜神经节细胞（RGC）类型之间先前未知的连接。这些新的联系通过生理记录得到了证实。VSV能够对神经回路进行细胞类型特异性解剖，并能揭示神经元之间的突触关系，否则由于神经细胞的复杂性和密度而被掩盖。
The use of neurotropic viruses as transsynaptic tracers was first described in the 1960s, but only recently have such viruses gained popularity as a method for labeling neural circuits. The development of retrograde monosynaptic tracing vectors has enabled visualization of the presynaptic sources onto defined sets of postsynaptic neurons. Here we describe the first application of a novel viral tracer, based on vesicular stomatitis virus (VSV), which directs retrograde transsynaptic viral spread between defined cell types. We use this virus in the mouse retina to show connectivity between starburst amacrine cells (SACs) and their known synaptic partners, direction selective retinal ganglion cells (DSGCs), as well as to discover previously unknown connectivity between SACs and other retinal ganglion cell (RGC) types. These novel connections were confirmed using physiological recordings. VSV enables cell type-specific dissection of neural circuitry and can reveal synaptic relationships among neurons that are otherwise obscured due to the complexity and density of neuropil.