Functional analysis of AeSCP-2 using gene expression knockdown in the yellow fever mosquito, Aedes aegypti
Functional analysis of AeSCP-2 using gene expression knockdown in the yellow fever mosquito, Aedes aegypti
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DOI：
10.1111/j.1365-2583.2005.00560.x
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发表时间：
2005-06-01
影响因子：
2.6
通讯作者：
Lan, Q
中科院分区：
文献类型：
--
作者：
Blitzer, EJ;Vyazunova, I;Lan, Q
利用基因表达敲低的效果来研究黄热病蚊子埃及伊蚊中甾醇载体蛋白-2 (AeSCP-2) 的功能。将小型双链 AeSCP-2 RNA 注射到蚊子幼虫中，导致基因产物被敲低。幼虫中缺乏 AeSCP-2 与蛹中积累的胆固醇减少同时发生，支持了 AeSCP-2 可能参与蚊子幼虫胆固醇摄取的假设。 AeSCP-2 的敲低会导致发育中成虫的高死亡率并降低卵子的活力。这项研究的结果表明，AeSCP-2 对于成体发育和卵的活力非常重要。
The effect of gene expression knockdown was used to study the function of the sterol carrier protein-2 (AeSCP-2) in the yellow fever mosquito, Aedes aegypti. Injection of small double stranded AeSCP-2 RNAs into mosquito larvae resulted in the knockdown of gene products. The lack of AeSCP-2 in larvae coincided with a reduction in accumulated cholesterol in pupae, supporting the hypothesis that AeSCP-2 may be involved in cholesterol uptake in mosquito larvae. Knockdown of AeSCP-2 caused a high mortality rate in developing adult and reduced egg viability. Results from this study indicate that AeSCP-2 is important for adult development and for the viability of the eggs.