Formation of functional CENP-B boxes at diverse locations in repeat units of centromeric DNA in New World monkeys.
Formation of functional CENP-B boxes at diverse locations in repeat units of centromeric DNA in New World monkeys.
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DOI：
10.1038/srep27833
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发表时间：
2016-06-13
影响因子：
4.6
通讯作者：
Koga A
中科院分区：
文献类型：
--
作者：
Kugou K;Hirai H;Masumoto H;Koga A
参与着丝粒形成的着丝粒蛋白B通过识别称为CENP-B盒的核苷酸基序与着丝粒重复DNA结合。人类在其常染色体和X染色体的着丝粒重复DNA中具有大量CENP-B盒。目前的理解是，这些CENP-B盒位于着丝粒DNA重复单元中的相同位置。类人猿也有CENP-B盒，位置与人类相同。本研究的目的是检查CENP-B盒在新世界猴中的位置。我们最近在一种新世界猴（绒猴）中发现了CENP-B盒。在这项研究中，我们通过免疫染色和ChIP-qPCR分析在另外2只新世界猴（松鼠猴和绢毛猴）的CENP-A组装的着丝粒DNA重复单位中发现了功能性CENP-B盒。重复单元中3个CENP-B盒的位置彼此不同。新世界猴的着丝粒DNA的重复单位大小（340-350 bp）大约是人类和类人猿（171 bp）的两倍。这可能与着丝粒DNA的高阶重复结构有关，是新世界猴CENP-B盒位置观察到变化的一个因素。
Centromere protein B, which is involved in centromere formation, binds to centromeric repetitive DNA by recognizing a nucleotide motif called the CENP-B box. Humans have large numbers of CENP-B boxes in the centromeric repetitive DNA of their autosomes and X chromosome. The current understanding is that these CENP-B boxes are located at identical positions in the repeat units of centromeric DNA. Great apes also have CENP-B boxes in locations that are identical to humans. The purpose of the present study was to examine the location of CENP-B box in New World monkeys. We recently identified CENP-B box in one species of New World monkeys (marmosets). In this study, we found functional CENP-B boxes in CENP-A-assembled repeat units of centromeric DNA in 2 additional New World monkeys (squirrel monkeys and tamarins) by immunostaining and ChIP-qPCR analyses. The locations of the 3 CENP-B boxes in the repeat units differed from one another. The repeat unit size of centromeric DNA of New World monkeys (340–350 bp) is approximately twice that of humans and great apes (171 bp). This might be, associated with higher-order repeat structures of centromeric DNA, a factor for the observed variation in the CENP-B box location in New World monkeys.