CAVEOLIN, A PROTEIN-COMPONENT OF CAVEOLAE MEMBRANE COATS
CAVEOLIN, A PROTEIN-COMPONENT OF CAVEOLAE MEMBRANE COATS
复制标题
DOI：
10.1016/0092-8674(92)90143-z
复制
发表时间：
1992-02-21
期刊：
影响因子：
64.5
通讯作者：
ANDERSON, RGW
中科院分区：
文献类型：
--
作者：
ROTHBERG, KG;HEUSER, JE;ANDERSON, RGW
小凹与大分子穿透内皮细胞和受体介导的5-甲基四氢叶酸摄取有关。结构研究表明，小窝由形成条纹涂层的独特的细丝或线束装饰在细胞质表面。为了了解这些非网状蛋白涂层凹坑的功能，我们对条纹涂层进行了结构分析，并寻找了涂层材料的分子成分(S)。用高盐洗涤不能去除被毛；然而，将膜暴露在胆固醇结合药物中会导致内陷的凹陷变平，条纹被解体。针对病毒转化的鸡胚胎成纤维细胞中22 kd的v-src酪氨酸激酶底物的抗体装饰着细丝，表明这种分子是毛皮的一个组成部分。我们已将这种分子命名为小窝。小凹是第三种与分子转运有关的涂层膜特化。
Caveolae have been implicated in the transcytosis of macromolecules across endothelial cells and in the receptor-mediated uptake of 5-methyltetrahydrofolate. Structural studies indicate that caveolae are decorated on their cytoplasmic surface by a unique array of filaments or strands that form striated coatings. To understand how these nonclathrin-coated pits function, we performed structural analysis of the striated coat and searched for the molecular component(s) of the coat material. The coat cannot be removed by washing with high salt; however, exposure of membranes to cholesterol-binding drugs caused invaginated caveolae to flatten and the striated coat to disassemble. Antibodies directed against a 22 kd substrate for v-src tyrosine kinase in virus-transformed chick embryo fibroblasts decorated the filaments, suggesting that this molecule is a component of the coat. We have named the molecule caveolin. Caveolae represent a third type of coated membrane specialization that is involved in molecular transport.