Predictability of the toxicity of multiple chemical mixtures to Vibrio fischeri:: Mixtures composed of similarly acting chemicals
Predictability of the toxicity of multiple chemical mixtures to Vibrio fischeri:: Mixtures composed of similarly acting chemicals
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DOI：
10.1002/etc.5620190926
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发表时间：
2000-09-01
影响因子：
4.1
通讯作者：
Grimme, LH
中科院分区：
文献类型：
--
作者：
Altenburger, R;Backhaus, T;Grimme, LH
通过使用浓度相加和独立作用的药理学概念，基于混合物中单个组分的效应预测组合效应可能是污染物混合物风险评估的一个有前途的工具。为了分析和比较类似作用化学品的参考概念的预测能力，在使用费氏弧菌的生物发光抑制测定中确定了多种混合物的总体毒性。该混合物由16种具有相似和特异性作用的化学品组成，预期通过氧化磷酸化的弱酸呼吸解偶联具有共同的作用模式。结果表明，观察到的混合物的毒性是相当好的预测这两个概念。浓度相加显示出极好的预测能力;预测混合物的中位有效浓度（EC 50），误差约为10%。相比之下，独立作用将混合物的EC 50低估了3倍多一点。关于风险评估程序，可以得出结论认为，在这类生物试验中，浓度相加可有效估计混合物成分具有相似和特定作用机制的多种混合物的总体毒性。
The prediction of combined effects based on the effects of the individual components of mixtures by using the pharmacological concepts of concentration addition and independent action might be a promising tool for the risk assessment of pollutant mixtures. To analyze and compare the predictive capabilities of the reference concepts for similarly acting chemicals, the overall toxicity of a multiple mixture was determined in a bioluminescence inhibition assay with Vibrio fischeri. The mixture was composed of 16 similarly and specifically acting chemicals, anticipated to have a common mode of action via weak acid respiratory uncoupling of oxidative phosphorylation. Results show that the observed mixture toxicity is rather well predicted by both concepts. Concentration addition shows an excellent predictive power; the median effective concentration (EC50) of the mixture is predicted with an error of about 10%. Independent action, in contrast, underestimates the EC50 of the mixture by a factor of a little more than three. With respect to risk assessment procedures, it may be concluded that concentration addition gives a valid estimation of the overall toxicity for multiple mixtures with similar and specific mechanisms of action of the mixture components in this type of biotest.