Systematic application of high-resolution, phase-sensitive two-dimensional 1H-NMR techniques for the identification of the amino-acid-proton spin systems in proteins. Rabbit metallothionein-2.
Systematic application of high-resolution, phase-sensitive two-dimensional 1H-NMR techniques for the identification of the amino-acid-proton spin systems in proteins. Rabbit metallothionein-2.
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系统应用高分辨率、相敏二维 1H-NMR 技术来鉴定蛋白质中的氨基酸-质子自旋系统。
DOI：
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Kurt Wüthrich
中科院分区：
文献类型：
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作者：
David Neuhaus;Gerhard Wagner;Milan Vašák;J. Kägi;Kurt Wüthrich
利用最近引入的二维NMR技术，如相敏双量子滤波相关光谱，中继相干转移光谱，双量子光谱和核Overhauser光谱，开发了新的策略，用于蛋白质中氨基酸1H-NMR自旋系统的阐明和分类。由于相敏光谱分辨率的提高，交叉峰的精细结构可以作为建立1H-1H连接性的有力信息来源。讨论了吸收模交叉峰多重态结构的解释原则。利用这些方法对兔肝金属硫蛋白-2的1H自旋系统进行了解析，并根据氨基酸类型进行了分类。尽管这种蛋白质的不寻常的氨基酸组成所产生的固有困难的情况下，一个更完整的表征的1H自旋系统的顺序共振分配的步骤之前，实现了与目前介绍的方法比可能在早期的研究类似大小的蛋白质。
Novel strategies for elucidation and classification of amino acid 1H-NMR spin systems in proteins were developed exploiting recently introduced two-dimensional NMR techniques such as phase-sensitive double-quantum-filtered correlated spectroscopy, relayed coherence transfer spectroscopy, double quantum spectroscopy and nuclear Overhauser spectroscopy. Due to the improved resolution in phase-sensitive spectra, the fine structure of cross peaks could be exploited as a powerful source of information for establishing 1H-1H connectivities. Principles for the interpretation of multiplet structures of absorption mode cross peaks are discussed. With these methods the 1H spin systems of rabbit liver metallothionein-2 were elucidated and classified according to amino acid types. Despite the intrinsically difficult situation arising from the unusual amino acid composition of this protein, a more complete characterization of the 1H spin systems prior to the step of sequential resonance assignments was achieved with the presently introduced methodology than was possible in earlier studies of proteins of similar size.
溶液中 apamin 的结构：二维核磁共振研究。
影响因子：
2.9
作者：
Wemmer,D;Kallenbach,NR
通讯作者：
Kallenbach,NR