Effect of a Combined Treatment With Growth Hormone and Melatonin in the Cardiological Aging on Male SAMP8 Mice
Effect of a Combined Treatment With Growth Hormone and Melatonin in the Cardiological Aging on Male SAMP8 Mice
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DOI：
10.1093/gerona/glr083
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发表时间：
2011-08-01
影响因子：
5.1
通讯作者：
Tresguerres, J. A. F.
中科院分区：
文献类型：
--
作者：
Forman, Katherine;Vara, Elena;Tresguerres, J. A. F.
研究了生长激素（GH）加褪黑激素（Mel）慢性联合治疗对SAMP8小鼠（2个月和10个月）心脏细胞质和核部分不同年龄相关过程的影响。研究的参数包括IL-1、IL-10、NFkBp50、NFkBp52、TNF α、eNOS、iNOS、HO-1、HO-2、BAD、BAX和Bcl2的信使RNA表达以及iNOS、eNOS、TNF α、IL-1、IL-10、NFkBp50、NFkBp52的蛋白表达和caspase活性（3和9）。我们的研究结果支持衰老小鼠心脏中存在促凋亡和氧化状态以及炎症过程，炎症因子、caspase活性、HO-1、BAX、NFkBp50和NFkBp52升高，eNOS和Bcl2降低。此外，我们还能够观察到NFkB向细胞核的易位。联合治疗能够部分减少这些有害变化的发生率，这与我们组以前的文章中研究的GH和Mel分开治疗有所不同。
The effect of a chronic combined treatment with growth hormone (GH) plus melatonin (Mel) on different age-related processes in cytosolic and nuclear fractions of hearts from SAMP8 mice (2 and 10 months) has been investigated. The parameters studied have been messenger RNA expressions of IL-1, IL-10, NFkBp50, NFkBp52, TNF alpha, eNOS, iNOS, HO-1, HO-2, BAD, BAX, and Bcl2 and protein expressions of iNOS, eNOS, TNF alpha, IL-1, IL-10, NFkBp50, NFKbp52, and caspase activity (3 and 9). Our results supported the existence of a proapoptotic and oxidative status together with inflammatory processes in the heart of old mice, with increases of inflammatory cytokines, caspase activity, HO-1, BAX, NFkBp50, and NFkBp52 and decreases of eNOS and Bcl2. Also, we were able to observe the translocation of NFkB to nuclei. The combined treatment was able to partially reduce the incidence of these deleterious changes, showing differences with the separated treatments with GH and Mel as were investigated in previous articles from our group.