Differential effects of Chlamydia pneumoniae infection on cytokine levels in human T lymphocyte- and monocyte-derived cell cultures
Differential effects of Chlamydia pneumoniae infection on cytokine levels in human T lymphocyte- and monocyte-derived cell cultures
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DOI：
10.1016/j.ijmm.2006.11.004
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发表时间：
2007-04-01
影响因子：
4.1
通讯作者：
Friedman, Herman
中科院分区：
文献类型：
--
作者：
Mamata, Yukimitsu;Hakki, Amal;Friedman, Herman
连续培养的人淋巴细胞和单核细胞来源的细胞系的免疫调节细胞因子的水平进行了检查，重要的抵抗细胞内的机会性细菌肺炎衣原体（Cp），一种普遍存在的病原体广泛传播的人口和假设参与慢性炎症性疾病，如动脉粥样硬化和神经系统疾病，如多发性硬化症和阿尔茨海默氏病。本研究的结果表明，连续的人T淋巴细胞系MOLT-4和连续的单核细胞系THP-1在体外容易被Cp感染，如Cp脂多糖（LPS）的免疫荧光显微镜所示。通过实时RT-PCR测定的16 S rRNA表达在用这些细菌感染任一细胞系后迅速增加。与用热灭活Cp（KCp）或作为对照的大肠杆菌LPS刺激的培养物相比，用Cp感染的THP-1细胞显示免疫调节细胞因子IL-12以及与IL-10成比例的TNF水平增加。用KCp或大肠杆菌LPS刺激MOLT-4细胞也诱导Th 1细胞因子IFN γ和IL-12以及Th 2细胞因子IL-10，但用活Cp感染诱导更高的Th 1细胞因子水平。这些结果表明，Cp感染除了通过单核细胞/巨噬细胞诱导TNF α外，还通过T细胞诱导主要的Thl细胞因子谱。这种作用可能涉及对Cp感染的抗菌免疫。(c)2007年由Elsevier GmbH出版。
Continuous cultures of human lymphocyte- and monocyte-derived cell lines were examined for levels of immunoregulatory cytokines important in resistance to the intracellular opportunistic bacterium Chlamydia pneumoniae (Cp), a ubiquitous pathogen widely disseminated in the population and hypothesized to be involved in chronic inflammatory diseases such as atherosclerosis and neurological diseases like multiple sclerosis and Alzheimer's disease. The results of this study showed that the continuous human T lymphocyte cell line MOLT-4 and the continuous monocytic cell line THP-1 were readily infected by Cp in vitro as shown by immunofluorescence microscopy for Cp lipopolysaccharide (LPS). The 16S rRNA expression determined by real-time RT-PCR increased rapidly after infection of either cell line with these bacteria. The THP-1 cells infected with Cp showed increased levels of the immunoregulatory cytokine IL-12 and also of TNF proportional to and IL-10 compared to cultures stimulated with heat-killed Cp (KCp) or Escherichia coli LPS as a control. Stimulation of MOLT-4 cells with KCp or E coli LPS also induced the Thl cytokines IFN gamma and 1L-12 and the Th2 cytokine IL-10, but infection with viable Cp induced higher Thl cytokine levels. These results suggest that Cp infection induces a predominant Thl cytokine profile by T cells, in addition to induction of TNF alpha by monocytes/macrophages. Such effects are likely involved in antibacterial immunity against Cp infection. (c) 2007 Published by Elsevier GmbH.