INTRACELLULAR BACTERIA OF PORCINE PROLIFERATIVE ENTEROPATHY - CULTIVATION AND MAINTENANCE INVITRO
INTRACELLULAR BACTERIA OF PORCINE PROLIFERATIVE ENTEROPATHY - CULTIVATION AND MAINTENANCE INVITRO
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DOI：
10.1128/jcm.31.5.1136-1142.1993
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发表时间：
1993-05-01
影响因子：
9.4
通讯作者：
MACKIE, RA
中科院分区：
文献类型：
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作者：
LAWSON, GHK;MCORIST, S;MACKIE, RA
从来自四个不同猪场的所有10例猪增殖性肠病的肠道中分离出一种专性细胞内细菌。该微生物在大鼠肠上皮细胞系（IEC-18）中生长，并保持超过20代。通过暴露于感染的细胞的免疫染色来评估细菌的生长。感染与细胞形态学变化无关，生长仅限于每次转移感染时感染的细胞和这些细胞的后代。该细菌是一种微需氧、细胞依赖性、弯曲或杆状、革兰氏阴性杆菌，在肠上皮细胞细胞质中自由繁殖。含有细胞内细菌的细胞培养物似乎不含其他微生物，包括衣原体和病毒。
An obligate intracellular bacterium was isolated from the intestines of all 10 cases of porcine proliferative enteropathy from four different pig farms. The organism grew in a rat enterocyte cell line (IEC-18) and was maintained over 20 passages. The growth of the bacteria was assessed by immunostaining of cells exposed to infection. Infection was not associated with morphological cell change, and growth was confined to cells infected at the time of each transfer of infection and the progeny of these cells. The bacterium is a microaerophilic, cell dependent, curved or rod-shaped, gram-negative bacillus that multiplies freely in the enterocyte cytoplasm. Cell cultures containing the intracellular bacteria appear to be free of other microorganisms, including chlamydiae and viruses.