EFFECTS OF 3-NITROBENZOTHIAZOLO[3,2-A]QUINOLINIUM CHLORIDE (NBQ) AND DOXORUBICIN ON LENS REGENERATION IN THE ADULT NEWT - A MORPHOLOGICAL-STUDY
EFFECTS OF 3-NITROBENZOTHIAZOLO[3,2-A]QUINOLINIUM CHLORIDE (NBQ) AND DOXORUBICIN ON LENS REGENERATION IN THE ADULT NEWT - A MORPHOLOGICAL-STUDY
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DOI：
10.1046/j.1432-0436.1994.5530169.x
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发表时间：
1994-02-01
期刊：
影响因子：
2.9
通讯作者：
BAEZ, A
中科院分区：
文献类型：
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作者：
HERRENOSAENZ, D;ORTIZ, JR;BAEZ, A
抗肿瘤药物3-硝基苯并噻唑[3,2-a]氯化喹啉（NBQ）刺激成年蝾螈（Notophthalmus viri下降）体内晶状体再生，诱导HL-60白血病细胞分化。由于细胞毒性药物阿霉素（阿霉素）在体外诱导HL-60细胞分化，我们决定比较阿霉素与NBQ对体内晶状体再生的影响。这两种药物以六种不同的时间表进行腹腔注射。采用不同再生阶段的形态学标准对再生体进行分析。NBQ刺激晶状体再生独立于给药的时间间隔和再生阶段。有丝分裂的平均数量增加，表明NBQ刺激了细胞增殖。给予5天的阿霉素没有改变再生过程。另一方面，在晶状体切除术后给予多柔比星9天或更长时间，可强烈抑制新晶状体的形成。因此，阿霉素的抑制作用依赖于与组织的持续长期接触。虽然NBQ和阿霉素都是DNA嵌入剂，但它们通过不同的机制诱导晶状体再生。
The antitumor drug 3-nitrobenzothiazolo [3,2-a]quinolinium chloride (NBQ) stimulates the in vivo lens regeneration in the adult newt Notophthalmus viridescens and induces a differentiated state in HL-60 leukemia cells. Because the cytotoxic drug doxorubicin (Adriamycin) induces differentiation of HL-60 cells in vitro we decided to compare the effect(s) of doxorubicin with NBQ on lens regeneration in vivo. Both drugs were injected intraperitoneally at six different schedules. Morphological criteria of the different regeneration stages were used in the analysis of the regenerates. NBQ stimulated lens regeneration independently of the time intervals and the stage of regeneration at which the drug was administered. There was an increase in the mean number of mitoses suggesting that NBQ stimulated cell proliferation. Doxorubicin administered for five days did not modify the regenerative process. On the other hand, doxorubicin given for periods of nine or more days after lentectomy, strongly inhibited the formation of a new lens. Thus, the inhibitory effect of doxorubicin is dependent on the continuous long term contact with the tissue. Although NBQ and doxorubicin are both DNA intercalators, they induced the effects on lens regeneration through different mechanisms.