PRESENCE OF EPIDERMAL GROWTH-FACTOR, PLATELET-DERIVED GROWTH-FACTOR, AND THEIR RECEPTORS IN HUMAN MYOMETRIAL TISSUE AND SMOOTH-MUSCLE CELLS - THEIR ACTION IN SMOOTH-MUSCLE CELLS-INVITRO
PRESENCE OF EPIDERMAL GROWTH-FACTOR, PLATELET-DERIVED GROWTH-FACTOR, AND THEIR RECEPTORS IN HUMAN MYOMETRIAL TISSUE AND SMOOTH-MUSCLE CELLS - THEIR ACTION IN SMOOTH-MUSCLE CELLS-INVITRO
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DOI：
10.1210/en.130.3.1716
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发表时间：
1992-03-01
期刊：
影响因子：
4.8
通讯作者：
MASTERSON, BJ
中科院分区：
文献类型：
--
作者：
ROSSI, MJ;CHEGINI, N;MASTERSON, BJ
免疫组织化学观察表明，人子宫肌层平滑肌细胞表达表皮生长因子（EGF）和血小板源性生长因子（PDGF）-AB，并含有EGF和PDGF-β受体，其强度随月经周期的阶段而无变化。 此外，免疫荧光显微镜研究显示，原代子宫肌层平滑肌细胞培养物也表达EGF，PDGF-AB，并含有EGF和PDGF-β，但不含α受体。 H-3-胸苷掺入和标记指数表明，在无血清培养基中孵育亚融合平滑肌细胞48小时内会导致静止。 将静止细胞暴露于10%胎牛血清以时间依赖性方式刺激DNA合成和增殖的恢复，倍增时间为41.6 h。 EGF（1.5-50 ng/ml）和PDGF-AB（1-10 ng/ml）均能显著促进静止期平滑肌细胞H-3-TdR掺入（P < 0.05），并呈剂量和时间依赖性。 EGF（15 ng/ml）和PDGF-AB（10 ng/ml）联合应用可显著增加任一生长因子单独诱导的H-3-胸苷掺入（P < 0.05）。 10 ng/ml的PDGF-BB也刺激H-3-胸苷掺入，其作用与相同浓度的PDGF-AB诱导的作用相似。 1 μ M的17-β-雌二醇（E2）抑制平滑肌细胞H-3-胸苷掺入（P < 0.05）。 E2还降低EGF（15 ng/ml）和PDGF（3 ng/ml）的刺激作用。 孕酮在1 μ M单独或与E2结合没有任何影响H-3-胸苷掺入或改变EGF和PDGF的促有丝分裂作用。 EGF和PDGF对子宫肌层平滑肌细胞的细胞生长和H-3-胸苷掺入的影响是独立的月经周期的阶段。总之，本研究的结果表明，人子宫肌层组织和子宫肌层平滑肌细胞在原代培养中局部产生EGF和PDGF-AB，并含有EGF和PDGF-beta，但不α-受体。 此外，培养的子宫肌层平滑肌细胞对EGF和PDGF的促有丝分裂作用有反应。
Immunohistochemical observations indicate that human myometrial smooth muscle cells express epidermal growth factor (EGF) and platelet-derived growth factor (PDGF)-AB and contain EGF and PDGF-beta receptors with no variation in intensity with phases of the menstrual cycle. Furthermore, immunofluorescent microscopic studies revealed that primary myometrial smooth muscle cell cultures also express EGF, PDGF-AB, and contain EGF and PDGF-beta, but not alpha-receptor. Incubation of subconfluent smooth muscle cells in serum-free medium leads to quiescence within 48 h as demonstrated by H-3-thymidine incorporation and labeling index. Exposure of quiescent cells to 10% fetal bovine serum stimulates resumption of DNA synthesis and proliferation in a time-dependent manner with a doubling time of 41.6 h. EGF (1.5-50 ng/ml) and PDGF-AB (1-10 ng/ml) in a dose- and time-dependent manner significantly stimulated H-3-thymidine incorporation by quiescent myometrial smooth muscle cells (P < 0.05). Combinations of EGF (15 ng/ml) and PDGF-AB (10 ng/ml) significantly increased H-3-thymidine incorporation induced by either growth factor alone (P < 0.05). PDGF-BB at 10 ng/ml also stimulated H-3-thymidine incorporation and its effect was similar to that induced by PDGF-AB at the same concentration. 17-beta-Estradiol (E2) at 1-mu-M inhibited H-3-thymidine incorporation by the smooth muscle cells (P < 0.05). E2 also reduced the stimulatory effect of EGF (15 ng/ml) and PDGF (3 ng/ml). Progesterone at 1-mu-M either alone or in combination with E2 did not have any effect on H-3-thymidine incorporation or alter the mitogenic action of EGF and PDGF. The effect of EGF and PDGF on cell growth and H-3-thymidine incorporation by myometrial smooth muscle cells was independent of phases of the menstrual cycle.In summary, the results of present studies indicate that human myometrial tissue and myometrial smooth muscle cells in primary culture locally produce EGF and PDGF-AB and contain EGF and PDGF-beta, but not alpha-receptors. Moreover, the myometrial smooth muscle cells in culture respond to the mitogenic action of EGF and PDGF.