Helium-Neon Laser Irradiation Promotes the Proliferation and Migration of Human Epidermal Stem Cells In Vitro: Proposed Mechanism for Enhanced Wound Re-epithelialization
Helium-Neon Laser Irradiation Promotes the Proliferation and Migration of Human Epidermal Stem Cells In Vitro: Proposed Mechanism for Enhanced Wound Re-epithelialization
复制标题
氦氖激光照射促进体外人表皮干细胞的增殖和迁移：增强伤口上皮再生的拟议机制
DOI：
10.1089/pho.2013.3667
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发表时间：
2014-04-01
影响因子：
--
通讯作者：
Fu, Xiao-Bing
中科院分区：
文献类型：
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作者：
Liao, Xuan;Xie, Guang-Hui;Fu, Xiao-Bing
目的：研究氦氖（He-Ne）激光照射对体外培养的人表皮干细胞（ESCs）增殖、迁移和分化的影响。背景资料：已知波长为632.8 nm的He-Ne激光器具有光生物学效应，广泛用于加速伤口愈合；然而，所涉及的细胞机制尚未完全了解。方法：以人包皮为材料制备ESCs，采用632.8 nm、2 J/cm(2)的He-Ne激光照射。分别采用XTT法、划痕法和流式细胞术检测ESC细胞的增殖、迁移和分化。Western blotting分析细胞外信号调节激酶（ERK）的磷酸化。结果：He-Ne激光照射可显著促进细胞增殖和迁移，并伴有ERK磷酸化升高，但对细胞分化无明显影响。结论：我们的数据表明，氦氖激光光刺激导致体外人ESC增殖和迁移显著增加，这可能至少部分地促进了低水平激光治疗加速伤口再上皮化。
Objective: The present study was conducted to investigate the effects of helium-neon (He-Ne) laser irradiation on the proliferation, migration, and differentiation of cultured human epidermal stem cells (ESCs). Background data: A He-Ne laser with a wavelength of 632.8 nm is known to have photobiological effects, and is widely used for accelerating wound healing; however, the cellular mechanisms involved have not been completely understood. Methods: The ESCs were prepared from human foreskin, and irradiated by using He-Ne laser at 632.8 nm with 2 J/cm(2). The ESC proliferation, migration, and differentiation were examined by using XTT assay, scratch assay, and flow cytometry technology, respectively. The phosphorylation of extracellular signal-regulated kinases (ERK) was analyzed by using Western blotting. Results: He-Ne laser irradiation markedly promoted cell proliferation and migration accompanied by an increase in the phosphorylation of ERK, but did not significantly influence cell differentiation. Conclusion: Our data indicated that photostimulation with a He-Ne laser resulted in a significant increase in human ESC proliferation and migration in vitro, which might contribute, at least partially, to accelerated wound re-epithelialization by low-level laser therapy.