TIME COURSE OF VERTEBRAL OSTEOPENIA IN OVARIECTOMIZED RATS
TIME COURSE OF VERTEBRAL OSTEOPENIA IN OVARIECTOMIZED RATS
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DOI：
10.1016/8756-3282(89)90067-7
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发表时间：
1989-01-01
期刊：
影响因子：
4.1
通讯作者：
HORNER, SL
中科院分区：
文献类型：
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作者：
WRONSKI, TJ;DANN, LM;HORNER, SL
在去卵巢（OVX）大鼠中，第一腰椎椎体松质骨组织的骨质减少变化是时间的函数。雌性Sprague Dawley大鼠（体重240 g， 90日龄）切除双侧卵巢或假手术，在卵巢切除后0-540天的不同时间处死。第一腰椎未经钙化处理，用于定量骨组织形态学测定。对照组大鼠松质骨体积保持相对稳定。40%在整个研究过程中。相比之下，OVX大鼠的松质骨体积在切除后180天中度减少至30-35%。OVX大鼠椎体骨质减少在晚些时候变得更加明显，松质骨体积下降到约0.1%。在180天到270天之间减少20%，并在整个研究期间保持在这个骨质减少水平。OVX大鼠35天时成骨细胞和破骨细胞表面高度升高，180天时逐渐向对照水平下降，270天时明显升高。OVX大鼠的矿化表面和骨形成率（组织水平，总表面参比）在35-70天最大限度地增加，随后下降到对照水平。然而，在270 - 540天期间，OVX大鼠与对照大鼠相比，这些参数仍然显著增加。在所有时间点，对照组和OVX大鼠的矿物质附着率几乎相同，并且在两组中随年龄线性下降。我们的研究结果表明，骨减少和骨转换增加发生在OVX大鼠腰椎椎体中，正如之前在这些动物的胫骨近端外骺端观察到的那样。然而，在OVX大鼠中，椎体骨质减少比胫骨骨质减少发展得更慢，也不那么明显。OVX大鼠骨质减少变化的时间特征可以作为设计OVX大鼠作为绝经后骨质流失动物模型的未来研究的基础。
Osteopenic changes in cancellous bone tissue of the first lumbar vertebral body were characterized in ovariectomized (OVX) rats as a function of time. Female Sprague Dawley rats (240 g body weight, 90 days old) were subjected to bilateral ovariectomy or sham surgery and sacrificed at various times from 0-540 days postovariectomy. The first lumbar vertebra was processed undecalcified for quantitative bone histomorphometry. Cancellous bone volume remained relatively constant in control rats at .apprx.40% throughout the duration of the study. In contrast, cancellous bone volume was moderately decreased to 30-35% in OVX rats out to 180 days postovariectomy. Vertebral osteopenia became more pronounced in OVX rats at later times as cancellous bone volume declined to .apprx.20% between 180 and 270 days and remained at that osteopenic level for the duration of the study. Osteoblast and osteoclast surface were highly elevated in OVX rats at 35 days, declined gradually toward control levels out to 180 days, then increased markedly at 270 days. Mineralizing surface and bone formation rate (tissue level, total surface referent) were maximally increased in OVX rats at 35-70 days before declining toward control levels at later times. However, these parameters remained significantly increased in OVX rats relative to control rats between 270 and 540 days. Mineral apposition rate was nearly identical in control and OVX rats at all time points and declined linearly with age in both groups. Our results indicate that osteopenia and increased bone turnover occur in the lumbar vertebral bodies of OVX rats, as had been previously observed in the proximal tibial metaphyses of these animals. However, vertebral osteopenia develops more slowly and is less pronounced than tibial osteopenia in OVX rats. This temporal characterization of osteopenic changes in OVX rats may serve as a basis for the design of future studies with the OVX rat as an animal model for postmenopasual bone loss.