Classification of Leptospira from the eyes of horses suffering from recurrent uveitis
Classification of Leptospira from the eyes of horses suffering from recurrent uveitis
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DOI：
10.1111/j.1439-0450.2004.00740.x
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发表时间：
2004-04-01
期刊：
影响因子：
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通讯作者：
Wollanke, B
中科院分区：
文献类型：
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作者：
Hartskeerl, RA;Goris, MGA;Wollanke, B
马复发性葡萄膜炎（ERU）的发病机制尚不完全清楚。致病性钩端螺旋体在眼睛的持续感染可能是主要原因。在本研究中，我们从以下西欧国家的501匹马的眼内样本中分离并鉴定了32.2%的钩体：德国、瑞士、奥地利、荷兰、卢森堡、英国、意大利和波兰。绝大多数钩端螺旋体分离株属于肺炎伤寒血清群（78.2%）。其余分离株分别为Australis群（14.2%）、Scjroe群（3.6%）、Pomona群（2.5%）和Javanica群（1.5%）。除了三株可能属于克氏钩端螺旋体的分离株。血清学中，所有分离株均属于克氏杆菌群，血清学中均为血清学。单克隆抗体分析显示，分离株与血清型伤寒参比株Moskva V不同。然而，分离的伤寒链球菌与从一名荷兰病人身上分离出的细菌性伤寒链球菌菌株Duyster非常相似。由于Moskva V型和Duyster型在表型和基因型上存在明显差异，我们在血清型中提出了Moskva型和Duyster型两种不同的类型。Duyster型的分布仅限于西欧。引人注目的是，只有少数血清型从患有ERU的马的眼睛中分离出来。没有发现其他一些常见的血清型，如哥本哈根血清型，而血液和眼液中存在针对这些血清型的抗体，表明发生了感染。因此，我们假设，虽然许多致病血清型可能能够穿透眼睛，但只有少数能够持续存在。
The pathogenesis of equine recurrent uveitis (ERU) is not completely understood. Persistent infection of pathogenic leptospires in the eyes is the probable primary cause. Consistently, we isolated and characterized in this study leptospires from 32.2% of the intraocular samples collected from 501 horses from the following Western European countries: Germany, Switzerland, Austria, The Netherlands, Luxembourg, Great Britain, Italy and Poland. The vast majority of the leptospiral isolates belonged to serogroup Grippotyphosa (78.2%). The other isolates belonged to the Australis group (14.2%), the Scjroe group (3.6%), the Pomona group (2.5%) and the Javanica group (1.5%). Apart from three isolates that likely belong to Leptospira kirschneri. serovar Dadas, all Grippotyphosa group isolates belonged to L. kirschneri, serovar Grippotyphosa. Analysis with monoclonal antibodies revealed that the isolates differed from Moskva V, the reference strain of serovar Grippotyphosa. However, the Grippotyphosa isolates closely resembled serovar Grippotyphosa, strain Duyster, which has been isolated from a Dutch patient. Because of apparent phenotypic and genotypic differences between the strains Moskva V and Duyster, we propose two different types within serovar Grippotyphosa, namely type Moskva and type Duyster. Distribution of type Duyster is restricted to Western Europe. Strikingly, only a few serovars were isolated from the eyes of horses with ERU. A number of other commonly occurring serovars, such as Copenhageni, were not found, whereas the presence of antibodies in blood and eye fluids against such serovars suggests that infection occurs. Therefore, we hypothesize that, while many pathogenic serovars might be able to penetrate the eyes, only few are capable to persist.