GhWRKY6 Acts as a Negative Regulator in Both Transgenic Arabidopsis and Cotton During Drought and Salt Stress
GhWRKY6 Acts as a Negative Regulator in Both Transgenic Arabidopsis and Cotton During Drought and Salt Stress
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GhWRKY6 在干旱和盐胁迫期间作为转基因拟南芥和棉花的负调节剂
DOI：
10.3389/fgene.2019.00392
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发表时间：
2019-04-26
影响因子：
3.7
通讯作者：
Yang, Zhaoen
中科院分区：
文献类型：
--
作者：
Li, Zhi;Li, Lei;Yang, Zhaoen
干旱和高盐是制约棉花生产的关键因素。因此，对棉花潜在的胁迫响应机制的研究日益受到关注。我们首先鉴定并克隆了一个编码棉花525个氨基酸的新基因GhWRKY6。qRT-PCR分析表明，GhWRKY6是NaCl、PEG 6000和ABA诱导的。发芽率和根长分析表明，GhWRKY6过表达导致拟南芥对ABA、NaCl和PEG 6000过敏。相比之下，在这些处理条件下，功能丧失突变体wrky6不敏感，根比野生型略长。此外，GhWRKY6在拟南芥中的过表达通过调节ABA信号通路调节盐和干旱敏感表型和气孔开度，并通过活性氧（ROS）的富集、脯氨酸含量的降低、电解质和丙二醛（MDA）的增加来降低植物对非生物胁迫的耐受性。ABA、盐和干旱相关标记基因在过表达幼苗中的表达水平发生改变。病毒诱导的基因沉默（VIGS）技术表明，GhWRKY6基因的下调提高了棉花的耐盐性。这些结果表明，GhWRKY6是通过ABA信号通路调控植物对非生物胁迫反应的负调控因子。
Drought and high salinity are key limiting factors for cotton production. Therefore, research is increasingly focused on the underlying stress response mechanisms of cotton. We first identified and cloned a novel gene encoding the 525 amino acids in cotton, namely GhWRKY6. qRT-PCR analysis indicated that GhWRKY6 was induced by NaCl, PEG 6000 and ABA. Analyses of germination rate and root length indicated that overexpression of GhWRKY6 in Arabidopsis resulted in hypersensitivity to ABA, NaCl, and PEG 6000. In contrast, the loss-of-function mutant wrky6 was insensitive and had slightly longer roots than the wild-type did under these treatment conditions. Furthermore, GhWRKY6 overexpression in Arabidopsis modulated salt- and drought-sensitive phenotypes and stomatal aperture by regulating ABA signaling pathways, and reduced plant tolerance to abiotic stress through reactive oxygen species (ROS) enrichment, reduced proline content, and increased electrolytes and malondialdehyde (MDA). The expression levels of a series of ABA-, salt- and drought-related marker genes were altered in overexpression seedlings. Virus-induced gene silencing (VIGS) technology revealed that down-regulation of GhWRKY6 increased salt tolerance in cotton. These results demonstrate that GhWRKY6 is a negative regulator of plant responses to abiotic stress via the ABA signaling pathway.