GMF is an Evolutionarily Developed ADF/Cofilin-Super Family Protein Involved in the Arp2/3 Complex-Mediated Organization of the Actin Cytoskeleton
GMF is an Evolutionarily Developed ADF/Cofilin-Super Family Protein Involved in the Arp2/3 Complex-Mediated Organization of the Actin Cytoskeleton
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DOI：
10.1002/cm.20451
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发表时间：
2010-06-01
期刊：
影响因子：
2.9
通讯作者：
Takaine, Masak
中科院分区：
文献类型：
--
作者：
Nakano, Kentaro;Kuwayama, Hidekazu;Takaine, Masak
肌动蛋白解聚因子（ADF）/cofilin在真核生物中广泛表达，并通过分解肌动蛋白丝而在肌动蛋白细胞骨架的重组中起核心作用。ADF同源结构域（ADF-H）在其他几种肌动蛋白调节蛋白中是保守的，如twinfilin，Abp 1/dreplatin和coactosin。虽然这些蛋白质通过ADF-H与肌动蛋白相互作用，但它们对肌动蛋白的作用并不完全相同。在这里，我们报告了一种新的ADF/cofilin超家族蛋白，GMF 1（胶质成熟因子样蛋白1），从裂殖酵母裂殖酵母。gmf 1是肌动蛋白斑块的一个组成部分，它位于细胞皮层上，是内吞作用所必需的，并且可能参与控制肌动蛋白斑块的分解，因为它的过度表达会减少它们。我们提供的证据表明，Gmf 1结合弱，如果在所有的肌动蛋白，但它与肌动蛋白相关蛋白（阿普）2/3复合物和抑制其功能，如促进肌动蛋白聚合和分支丝。重要的是，Arp 2/3复合物抑制活性在来自其他生物体的GMF家族蛋白中是保守的。鉴于ADF-H的功能可塑性，GMF家族蛋白可能通过分子进化改变了其靶点。(C)2010 Wiley-Liss，Inc
Actin-depolymerizing factor (ADF)/cofilin is widely expressed in eukaryotes and plays a central role in reorganizing the actin cytoskeleton by disassembling actin filaments. The ADF-homologous domain (ADF-H) is conserved in several other actin-modulating proteins such as twinfilin, Abp1/drebrin, and coactosin. Although these proteins interact with actin via ADF-H, their effects on actin are not identical to each other. Here, we report a novel ADF/cofilin-super family protein, Gmf1 (Glia maturation factor-like protein 1), from the fission yeast Schizosaccharomyces pombe. Gmf1 is a component of actin patches, which are located on the cell cortex and required for endocytosis, and may be involved in the control of the disassembly of actin patches since its overexpression diminishes them. We provide evidence that Gmf1 binds weakly if at all to actin, but it associates with actin-related protein (Arp) 2/3 complex and suppresses its functions such as the promotion of actin polymerization and branching filaments. Importantly, Arp2/3 complex-suppressing activity is conserved among GMF-family proteins from other organisms. Given the functional plasticity of ADF-H, GMF-family proteins possibly have changed their target from conventional actin Arps through molecular evolution. (C) 2010 Wiley-Liss, Inc