Weaning is associated with an upregulation of expression of inflammatory cytokines in the intestine of piglets
Weaning is associated with an upregulation of expression of inflammatory cytokines in the intestine of piglets
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DOI：
10.1093/jn/134.3.641
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发表时间：
2004-03-01
影响因子：
4.2
通讯作者：
Oswald, IP
中科院分区：
文献类型：
--
作者：
Pié, S;Lallès, JP;Oswald, IP
细胞因子在免疫细胞反应中发挥核心作用，但它们也参与组织完整性的维持。断奶时猪肠道细胞因子网络可能会发生变化，因为饮食和环境因素的突然变化会导致肠道发生重要的形态和功能适应。本研究使用 RT-PCR 测量了 28 日龄仔猪 (n = 45) 在断奶后不同时间点 (0、1、2、5 和 8 d) 沿小肠 (SI) 和近端结肠的 6 种炎症细胞因子的基因表达。还检查了 SI 中的绒毛隐窝结构以及乳糖酶和蔗糖酶的酶活性。结果证实断奶与 SI 的形态和酶促变化有关。此外，数据表明肠道细胞因子反应可分为两个阶段：早期急性反应（断奶后0至2天）和晚期持久反应（断奶后2至8天）。第 0 天和第 2 天之间，IL-1β、IL-6 和 TNF-α 信使 RNA (mRNA) 的水平增加。 IL-1β mRNA 的显着上调发生在肠道的大部分部位，而 IL-6 和 TNF-α mRNA 仅在肠道的特定部位显着增加。第2天至第8天之间，IL-1β、IL-6和TNF-α mRNA水平迅速恢复至断奶前值，但远端SI中TNF-α mRNA水平仍然较高。与第 0 天相比，IL-12 亚基 p40 (IL-12p40) 和 IL-18 mRNA 的水平也有所下降。总而言之，这些结果表明仔猪断奶与肠道炎症细胞因子基因表达的早期和短暂反应相关。
Cytokines play a central role in immune cell response, but they also participate in the maintenance of tissue integrity. Changes in the cytokine network of the pig gut may be expected at weaning, because abrupt changes in dietary and environmental factors lead to important morphological and functional adaptations in the gut. This study measured the gene expression of 6 inflammatory cytokines along the small intestine (SI) and the proximal colon in 28-d-old piglets (n = 45) at different time points (0, 1, 2, 5 and 8 d) postweaning, using RT-PCR. Villus-crypt architecture and enzymatic activities of lactase and sucrase in the SI were also examined. The results confirmed that weaning is associated with morphological and enzymatic changes in the SI. In addition, the data indicated that cytokine response in the gut could be divided into two periods: an early acute response (0 to 2 d postweaning) and a late long-lasting response (2 to 8 d postweaning). Between d 0 and d 2, the levels of IL-1beta, IL-6, and TNF-alpha messenger RNA (mRNA) increased. Marked upregulation of IL-1beta mRNA occurred in most parts of the intestine, whereas IL-6 and TNF-alpha mRNA markedly increased only at specific sites in the intestine. Between d 2 and d 8, the levels of IL-1beta, IL-6, and TNF-alpha mRNA rapidly returned to preweaning values, except that the level of TNF-alpha mRNA remained high in the distal SI. Levels of IL-12 subunit p40 (IL-12p40) and IL-18 mRNA also decreased, compared to those on d 0. Taken together, these results demonstrate that weaning in piglets is associated with an early and transient response in gene expression of inflammatory cytokines in the gut.