Mice that lack astrotactin have slowed neuronal migration.
Mice that lack astrotactin have slowed neuronal migration.
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DOI：
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发表时间：
2002-02
期刊：
影响因子：
4.6
通讯作者：
N. Adams;T. Tomoda;M. Cooper;G. Dietz;M. Hatten
中科院分区：
文献类型：
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作者：
N. Adams;T. Tomoda;M. Cooper;G. Dietz;M. Hatten
在发育过程中，由于前体细胞沿神经胶质定向迁移，大脑皮层区域形成层压。在此之前，我们已经使用了一种分析系统来鉴定星形胶质蛋白作为神经配体在神经胶质纤维上的迁移。为了在体内检测星形胶质细胞的功能，我们通过靶向基因破坏产生了一个零突变。阿司他汀无效小鼠的小脑比野生型小约10%。体外和体内小脑颗粒细胞分析显示浦肯野细胞的神经元-胶质结合减少，迁移率降低和异常发育。其后果是平衡性和协调性变差。因此，星形胶质蛋白在体内沿胶质过程迁移中起作用，这是产生层流结构和突触伴侣系统发育所必需的过程。
The cortical regions of the brain are laminated as a result of directed migration of precursor cells along glia during development. Previously, we have used an assay system to identify astrotactin as a neuronal ligand for migration on glial fibers. To examine the function of astrotactin in vivo, we generated a null mutation by targeted gene disruption. The cerebella of astrotactin null mice are approximately 10% smaller than wild type. In vitro and in vivo cerebellar granule cell assays show a decrease in neuron-glial binding, a reduction in migration rates and abnormal development of Purkinje cells. Consequences of this are poorer balance and coordination. Thus, astrotactin functions in migration along glial processes in vivo, a process required for generating laminar structures and for the development of synaptic partner systems.