Capsaicin receptor expression in the rat temporomandibular joint
Capsaicin receptor expression in the rat temporomandibular joint
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DOI：
10.1007/s00441-006-0183-7
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发表时间：
2006-07-01
影响因子：
3.6
通讯作者：
Tanaka, Teruo
中科院分区：
文献类型：
--
作者：
Ioi, Hideki;Kido, Mizuho A.;Tanaka, Teruo
在实验中，颞下颌关节（TMJ）神经单位对辣椒素有反应，辣椒素在临床上用于治疗TMJ疼痛。然而，辣椒素受体在TMJ中的存在以前没有被清楚地证明。免疫组织化学分析显示，颞下颌关节的神经和滑膜衬里细胞中存在瞬时受体电位香草酸亚型1（TRPV 1）表达。TRPV 1免疫反应神经分布在关节囊的滑膜中，并为关节室提供分支。椎间盘周边由TRPV 1神经供应，TRPV 1神经主要与小动脉相关，偶尔神经穿透滑膜衬里层。双重免疫荧光显示，许多TRPV 1免疫反应性神经被标记的神经肽降钙素基因相关肽，而很少被标记的IB 4-凝集素。本研究结果为TRPV 1在神经和滑膜衬里细胞中的存在提供了证据，因此TRPV 1可能参与了TMJ的伤害感受和炎症机制。
Experimentally, temporomandibular joint (TMJ) nerve units respond to capsaicin, which is used clinically to treat TMJ pain. However, the existence of capsaicin receptors in the TMJ has not previously been clearly demonstrated. Immunohistochemical analysis has revealed the presence of transient receptor potential vanilloid subtype 1 (TRPV1) expression in the nerves and synovial lining cells of the TMJ. TRPV1-immunoreactive nerves are distributed in the synovial membrane of the joint capsule and provide branches to the joint compartment. The disc periphery is supplied by TRPV1 nerves that are mostly associated with small arterioles, and occasional nerves penetrate to the synovial lining layer. Double immunofluorescence has shown that many TRPV1-immunoreactive nerves are labeled with neuropeptide calcitonin gene-related peptide, whereas few are labeled with IB4-lectin. The results provide evidence for the presence of TRPV1 in both nerves and synovial lining cells, which might thus be involved in the mechanism of nociception and inflammation in the TMJ.