CD8- dendritic cells preferentially cross-present Saccharomyces cerevisiae antigens
CD8- dendritic cells preferentially cross-present Saccharomyces cerevisiae antigens
复制标题
DOI：
10.1002/eji.200737647
复制
发表时间：
2008-02-01
影响因子：
5.4
通讯作者：
den Haan, Joke M. M.
中科院分区：
文献类型：
--
作者：
Backer, Ronald;van Leeuwen, Fred;den Haan, Joke M. M.
小鼠脾脏树突状细胞（DC）亚群具有不同的抗原提呈能力。CD 8（+）DC专门将抗原交叉呈递给CD 8（+）T细胞，而CD 8（-）DC在将抗原呈递给CD 4（+）T细胞方面更有效。在这项研究中，我们检测了CD 8（+）和CD 8（-）DC亚群分别将MHC I类和II类分子中的真菌抗原呈递给CD 8（+）和CD 4（+）T细胞的能力。我们使用表达卵清蛋白的酿酒酵母（酵母-OVA）作为真菌模型系统。CD 8（+）和CD 8（-）DC亚群均以等量吞噬酵母，并通过dectin-1介导摄取。此外，两种DC亚群在与酵母-OVA孵育后诱导相似的OVA特异性CD 4（+）T细胞增殖。然而，OVA特异性CD 8（+）T细胞活化的诱导主要限于CD 8（-）DC亚群。此外，只有CD 8（-）DC产生细胞因子，如IL-10和TNF-α，并增加IL-23 p19和IL-23 p40 mRNA水平响应酵母。我们的研究结果强烈表明，DC亚群在体内诱导适应性免疫应答中具有不同的功能。
Mouse splenic dendritic cell (DC) subsets possess distinct antigen-presentation abilities. CD8(+) DC are specialized in cross-presentation of antigens to CD8(+) T cells, whereas CD8(-) DC are more efficient in antigen presentation to CD4(+) T cells. In this study, we examined the capacity of CD8(+) and CD8(-) DC subsets to present fungal antigens in MHC class I and II molecules to CD8(+) and CD4(+) T cells, respectively. We used ovalbumin-expressing Saccharomyces cerevisiae (yeast-OVA) as a fungal model system. Both CD8(+) and CD8(-) DC subsets phagocytosed yeast in equal amounts and uptake was mediated via dectin-1. In addition, both DC subsets induced similar OVA-specific CD4(+) T cell proliferation after incubation with yeast-OVA. However, the induction of OVA-specific CD8(+) T cell activation was largely restricted to the CD8(-) DC subset. Furthermore, only CD8(-) DC produced cytokines such as IL-10 and TNF-alpha and increased IL-23p19 and IL-23p40 mRNA levels in response to yeast. Our results strongly suggest that DC subsets have different functions in the elicitation of adaptive immune responses in vivo.