Improved selectivity for the analysis of maternal serum and cord serum for polyfluoroalkyl chemicals
Improved selectivity for the analysis of maternal serum and cord serum for polyfluoroalkyl chemicals
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DOI：
10.1016/j.chroma.2010.10.051
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发表时间：
2011-04-15
影响因子：
4.1
通讯作者：
Calafat, Antonia M.
中科院分区：
文献类型：
--
作者：
Kato, Kayoko;Basden, Brian J.;Calafat, Antonia M.
全氟辛烷磺酸（PFOS）和全氟辛酸是研究最广泛的两种多氟烷基化学品（PFC），可以穿过胎盘。因此，需要关于幼儿接触全氟化学品的数据，以评估与此类接触相关的潜在健康影响。人类血清，特别是从孕妇和脐带血中采集的血清，可能含有内源性成分，这些成分会干扰高效液相色谱法从其他血清生物分子中分离全氟辛烷磺酸和另一种相关全氟辛烷磺酸（PFHxS）。此类干扰的存在可能会妨碍对脐带血清或孕妇血清中的全氟辛烷磺酸和全氟辛烷磺酸xS进行充分定量，并可能妨碍利用生物监测评估妊娠期接触这些重要全氟辛烷磺酸的情况。我们已经修改了我们的在线固相萃取-HPLC-同位素稀释-串联质谱分析方法用于测量血清中的全氟化碳，并开发了一种方法，允许消除这些潜在的干扰，而不影响分析灵敏度和吞吐量。将乙腈作为HPLC移动的相有机溶剂和Betasil C8 HPLC柱相结合，可最好地将全氟辛烷磺酸和全氟硫化氢与平行峰分离。除了消除这些干扰，乙腈方法具有更短的运行时间，是更敏感的大多数全氟化学品（检测限为0.1纳克/毫升，除全氟辛烷磺酸（0.2纳克/毫升））比我们以前的方法，使用甲醇的HPLC分离。本方法应提高准确性和选择性分析的母血和脐带血中的PFC。(C)出版社：Elsevier B. V.
Perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid, two of the most widely studied polyfluoroalkyl chemicals (PFCs), can cross the placenta. Therefore, data on the exposure to PFCs of the very young are needed to evaluate the potential health effects associated with such exposure. Human serum, especially serum collected from pregnant women and cord serum, may contain endogenous components that can interfere in the separation by high performance liquid chromatography (HPLC) of PFOS and another PFC of interest, perfluorohexane sulfonic acid (PFHxS), from other serum biomolecules. The presence of such interferences may prevent the adequate quantification of PFOS and PFHxS in cord serum or serum collected from pregnant women, and potentially hinder the assessment of gestational exposure to these important PFCs using biomonitoring. We have modified our on-line solid phase extraction-HPLC-isotope dilution-tandem mass spectrometry analytical method for measuring PFCs in serum and developed an approach that allows for the elimination of these potential interferences without compromising analytical sensitivity and throughput. The combination of acetonitrile as the HPLC mobile phase organic solvent and a Betasil C8 HPLC column provided the best separation of PFOS and PFHxS from interferent peaks. In addition to eliminating these interferences, the acetonitrile method has a shorter runtime and is more sensitive for most PFCs (limits of detection were 0.1 ng/mL except for PFOS (0.2 ng/mL)) than our previous method that used methanol for the HPLC separation. The present method should improve the precise and selective analysis of maternal and cord serum for PFCs. (C) 2010 Published by Elsevier B.V.