Synthesis of Post-translational Modifier Protein NEDD8 via Ligation of Peptide Hydrazides
Synthesis of Post-translational Modifier Protein NEDD8 via Ligation of Peptide Hydrazides
复制标题
通过肽酰肼连接合成翻译后修饰蛋白 NEDD8
DOI：
10.6023/cjoc201605013
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发表时间：
2016
影响因子：
1.9
通讯作者：
Li Yiming
中科院分区：
文献类型：
--
作者：
Guan Chaojian;Wang Tao;Wang Jun;Li Yiming
NEDD 8（neural precursor cell expressed developmentally downregulated 8）是真核生物中一种重要的泛素样修饰蛋白，参与调节细胞内一系列重要的生命过程。目前，获得NEDD 8的主要方法是重组蛋白表达。然而，产率相对较低，并且用于纯化的重组标签需要在额外的步骤中去除。本研究首次采用高温固相肽合成（SPPS）结合一锅法连接-脱硫策略合成了NEDD 8蛋白。该方法为进一步研究NEDD 8修饰蛋白奠定了基础。
As an important ubiquitin-like modifier protein in eukaryotic organisms, NEDD8 (neural precursor cell expressed developmentally down-regulated 8) is involved in regulating a series of important life processes in cells. Nowadays, the major method for obtaining NEDD8 is recombinant protein expression. However, the yield is relatively low and the recombined tag for purification needs to be removed in an extra step. In present work, NEDD8 protein was first synthesized in homogeneity by using high temperature assisted solid-phase peptide synthesis (SPPS) combining with the one-pot ligation-desulfurization strategy. This method lays the foundation for the study of NEDD8 modified proteins in future.