Potential variables affecting the quality of animal studies regarding pathophysiology of traumatic spinal cord injuries
Potential variables affecting the quality of animal studies regarding pathophysiology of traumatic spinal cord injuries
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DOI：
10.1038/sc.2015.215
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发表时间：
2016-08-01
期刊：
影响因子：
2.2
通讯作者：
Rahimi-Movaghar, V.
中科院分区：
文献类型：
--
作者：
Hassannejad, Z.;Sharif-Alhoseini, M.;Rahimi-Movaghar, V.
研究设计：这是一项Delphi研究。目的：确定可能影响创伤性脊髓损伤(TSCI)病理生理学动物研究结果的变量。地点：本研究在伊朗进行。方法：采用改良的两轮Delphi研究。作为第一轮，在文献和一系列焦点小组讨论的基础上编制了初步问卷。在第二轮中，参与者被要求通过10分制给这些项目打分。通过以下标准达成共识：(1)得分中位数必须达到7.5或更高，以及(2)至少70%的参与者需要评级为7或更高。结果：21位专家参与了本研究。从第一轮研究开始，制定了一份包含47个项目的清单。通过考虑上述就极其重要的因素达成共识的标准，我们得出了一份15项清单，包括物种、品系、损伤方法和程度、对照组、遗传背景、损伤严重程度、磨损、适当测试的使用、失明、治疗分配方法、规则和道德、年龄/体重、膀胱表达、动物/群体数量和统计数据。评分员之间的信度为Kappa=0.82(P
Study design: This is a Delphi study.Objectives: Defining variables that potentially influence the outcomes of an animal study regarding pathophysiology of traumatic spinal cord injury (TSCI).Setting: This study was conducted in Iran.Methods: A modified two-round Delphi study was conducted. As the first round, an initial questionnaire was developed on the basis of literature and a series of focus group discussions. In the second round, the participants were asked to score the items through a 10-point scale. Consensus was achieved through the following criteria: (1) the median of scores has to be at 7.5 or higher, and (2) at least 70% of participants need to rate 7 or higher. Also, the inter-rater reliability analysis was performed to determine consistency among raters using the Kappa coefficient and Cronbach's alpha.Results: Twenty-one experts participated in our study. From the first round of the study, a 47-item checklist was developed. By considering the aforementioned criteria for consensus building on extremely important factors, we reached a 15-item checklist including species, strain, method and level of injury, control group, genetic background, severity of injury, attrition, use of appropriate test, blindness, method of allocation to treatments, regulation and ethics, age/weight, bladder expression, number of animals/group and statistics. The inter-rater reliability for the raters was found to be Kappa=0.82 (P