DISTRIBUTION OF PECTIC POLYSACCHARIDES THROUGHOUT WALLS OF SUSPENSION-CULTURED CARROT CELLS - AN IMMUNOCYTOCHEMICAL STUDY
DISTRIBUTION OF PECTIC POLYSACCHARIDES THROUGHOUT WALLS OF SUSPENSION-CULTURED CARROT CELLS - AN IMMUNOCYTOCHEMICAL STUDY
复制标题
DOI：
10.1007/bf01384453
复制
发表时间：
1992-01-01
期刊：
影响因子：
2.9
通讯作者：
VANCUTSEM, P
中科院分区：
文献类型：
--
作者：
LINERS, F;VANCUTSEM, P
用识别多聚半乳糖醛酸构象表位的单克隆抗体（2F4）对悬浮培养的胡萝卜（D。carota L.）细胞在电子显微镜水平。在“年轻”或“成熟”细胞的小菌落中，多聚半乳糖醛酸基本上位于细胞或内衬细胞间隙之间的三通连接处扩展的中间层材料上，但未发现在初生壁中。远离连接区和细胞间隙的中层未被识别。在所有初生细胞壁内存在Larvinyl酯化的果胶聚合物，其仅在通过果胶甲基酯酶进行网格上脱酯化处理后由2个F4抗体检测到。高尔基体和相关的囊泡也仅在脱酯化处理后才被2F4抗体标记，这表明果胶聚合物以高度酯化的形式合成和分泌。在“衰老”细胞中观察到果胶酯化的减少，这可能是由于初级壁的果胶聚合物的原位酶促脱酯化。这些结果进行了讨论，在有关的生化分析表明，在细胞壁生长的果胶甲酯含量的变化。
A monoclonal antibody (2 F 4) recognizing a conformational epitope of polygalacturonic acid was used for immunogold localization of pectins in walls of suspension-cultured carrot (D. carota L.) cells at the electron microscopic level. In microcolonies of "young" or "mature" cells, polygalacturonic acid was essentially located on the middle lamella material expanded at three-way junctions between cells or lining intercellular spaces but was not found in primary walls. Middle lamellae far from junction zones and intercellular spaces were not recognized. Largely esterified pectic polymers, only detected by the 2 F 4 antibodies after on-grid de-esterification treatment by pectin methyl esterases, were present within all primary cell walls. Golgi bodies and associated vesicles were also labeled by the 2 F 4 antibodies only after de-esterification treatment, which indicates that pectic polymers are synthesized and secreted in a highly esterified form. A decrease of pectin esterification, which results probably from an in situ enzymatic de-esterification of the pectic polymers of the primary walls, was observed in "senescent" cells. These results are discussed in relation to biochemical analyses showing changes of the methyl ester content of pectins during the cell-wall growth.