Neuronal activity regulates the developmental expression and subcellular localization of cortical BDNF mRNA isoforms in vivo
Neuronal activity regulates the developmental expression and subcellular localization of cortical BDNF mRNA isoforms in vivo
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DOI：
10.1016/j.mcn.2004.11.010
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发表时间：
2005-03-01
影响因子：
3.5
通讯作者：
Domenici, L
中科院分区：
文献类型：
--
作者：
Pattabiraman, PP;Tropea, D;Domenici, L
BDNF表达的活动依赖性变化与发育可塑性有关。虽然它的表达广泛存在于视皮层，但其不同的转录本通过视觉经验的发育调控还没有被研究。在这里，我们调查了不同的BDNF转录本在大鼠出生后发育过程中的视皮层细胞表达。我们发现，转录本I和II只在成人中表达，但III和IV从出生后早期阶段表达。总BDNF mRNA在整个年龄组中表达。转录本III和IV显示出差异的细胞内定位，而前者仅在细胞体中检测到，后者存在于细胞体和树突状过程中。视觉活动的抑制降低了外显子的水平，外显子IV转录几乎从树突消失。体外实验也证实了上述结果，表明不同BDNF启动子在发育中的视皮层中具有特定的时间和细胞表达模式的活性依赖性调节。(c)2004年爱思唯尔公司All rights reserved.
Activity-dependent changes in BDNF expression have been implicated in developmental plasticity. Although its expression is widespread in visual cortex, developmental regulation of its different transcripts by visual experience has not been investigated. Here, we investigated the cellular expression of different BDNF transcripts in rat visual cortex during postnatal development. We found that transcripts I and II are expressed only in adults but III and IV are expressed from early postnatal stage. Total BDNF mRNA is expressed throughout the age groups. Transcripts III and IV show a differential intracellular localization, while former was detected only in cell bodies, latter is present both in cell bodies and dendritic processes. Inhibition of visual activity decreases the levels of exons, with exon IV transcript almost disappearing from dendrites. In vitro experiments also confirmed the above results, indicating activity-dependent regulation of different BDNF promoters with specific temporal and cellular patterns of expression in developing visual cortex. (c) 2004 Elsevier Inc. All rights reserved.