Protein S100-A7 Derived from Digested Dentin Is a Critical Molecule for Dentin Pulp Regeneration
Protein S100-A7 Derived from Digested Dentin Is a Critical Molecule for Dentin Pulp Regeneration
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DOI：
10.3390/cells8091002
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发表时间：
2019-09-01
期刊：
影响因子：
6
通讯作者：
Hayashi, Mikako
中科院分区：
文献类型：
--
作者：
Komichi, Shungo;Takahashi, Yusuke;Hayashi, Mikako
牙本质由无机硬组织和有机牙本质基质组成。dmc中含有多种生物活性分子，其中部分活性分子可被龋区内源性基质金属蛋白酶（MMPs）消化后释放。据报道，MMP20诱导的消化dmc可促进牙髓伤口愈合过程，但释放的关键分子负责这一现象尚不清楚。在这里，我们通过综合蛋白质组学方法和随后的大鼠磨牙牙髓盖盖实验，确定了S100-A7蛋白是消化DMCs牙髓愈合的关键分子。此外，免疫组织化学结果表明，S100-A7和晚期糖基化终产物受体（RAGE）作为S100-A7受体在牙髓愈合早期以及cd146阳性干细胞在损伤牙髓内的积累过程中具有特异性分布。我们的研究结果表明，MMP20从牙本质释放的S100-A7蛋白可能在牙本质牙髓再生中起关键作用。
Dentin consists of inorganic hard tissue and organic dentin matrix components (DMCs). Various kinds of bioactive molecules are included in DMCs and some of them can be released after digestion by endogenous matrix metalloproteinases (MMPs) in the caries region. Digested DMCs induced by MMP20 have been reported to promote pulpal wound healing processes, but the released critical molecules responsible for this phenomenon are unclear. Here, we identified protein S100-A7 as a critical molecule for pulpal healing in digested DMCs by comprehensive proteomic approaches and following pulp capping experiments in rat molars. In addition, immunohistochemical results indicated the specific distribution of S100-A7 and receptor for advanced glycation end-products (RAGE) as receptor for S100-A7 in the early stage of the pulpal healing process, and following accumulation of CD146-positive stem cells in wounded pulp. Our findings indicate that protein S100-A7 released from dentin by MMP20 might play a key role in dentin pulp regeneration.