Characterization and fine-mapping of a novel premature leaf senescence mutant yellow leaf and dwarf 1 in rice
Characterization and fine-mapping of a novel premature leaf senescence mutant yellow leaf and dwarf 1 in rice
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DOI：
10.1016/j.plaphy.2016.11.012
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发表时间：
2017-02-01
影响因子：
6.5
通讯作者：
Ma, Bingtian
中科院分区：
文献类型：
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作者：
Deng, Luchang;Qin, Peng;Ma, Bingtian
叶片是产生光合作用产物的主要器官。叶片衰老促进了光合作用产物和养分从源向库的转移，这对植物的发育特别是对作物产量是重要的。然而，叶片衰老的分子机制尚不清楚。在这里，我们发现了一个突变体，黄叶和矮秆1号(Yldl)，它表现出株高降低和叶片过早衰老。硝基蓝四氮唑和二氨基联苯胺染色分析表明，YYDL叶片中的活性氧含量高于野生型叶片。Yd1的光合色素含量显著降低。叶绿体崩解，充满异常的淀粉颗粒。结合批量分离和MutMap基因作图方法，将导致yld1表型的突变定位在73Mb着丝粒区域，并在该区域发现了位于3个新基因的3个非同义单核苷酸多态。3个候选基因的表达谱表明，LOC_0s06g29380具有最大的功能验证潜力。植物激素测定表明，与野生型叶片相比，ydl叶片中水杨酸的积累较高，并且ydl叶片对水杨酸的敏感性高于野生型叶片。这项工作为理解叶片衰老的分子调控机制奠定了基础，并可能揭示与叶片衰老相关的分子途径、淀粉代谢和水杨酸信号转导之间的新联系。(C)2016年爱思唯尔·马森SAS。版权所有。
Leaves are the main organs in which photosynthates are produced. Leaf senescence facilitates the translocation of photosynthates and nutrients from source to sink, which is important for plant development and especially for crop yield. However, the molecular mechanism of leaf senescence is unknown. Here, we identified a mutant, yellow leaf and dwarf 1 (yldl), which exhibited decreased plant height and premature leaf senescence. Nitroblue tetrazolium and diamiobenzidine staining analyses revealed that the concentrations of reactive oxygen species were higher in yldl leaves than in wild type leaves. The photosynthetic pigment contents were significantly decreased in yldl. The yldl chloroplasts had collapsed and were filled with abnormal starch granules. Combining bulk segregant and MutMap gene mapping approaches, the mutation responsible for the yldl phenotype was mapped to a 73 Mb centromeric region, and three non -synonymous single nucleotide polymorphisms located in three novel genes were identified in this region. The expression patterns of the three candidate genes indicated that LOC_0s06g29380 had the most potential for functional verification. Plant hormone measurements showed that salicylic acid was highly accumulated in yldl leaves when compared with wild type leaves, and yldl was more sensitive to salicylic acid than wild type. This work lays the foundation for understanding the molecular regulatory mechanism of leaf senescence, and may reveal new connections among the molecular pathways related to leaf senescence, starch metabolism and salicylic acid signaling. (C) 2016 Elsevier Masson SAS. All rights reserved.