Estimating sample sizes for DNA barcoding
Estimating sample sizes for DNA barcoding
复制标题
估计 DNA 条形码的样本量。
DOI：
10.1016/j.ympev.2009.09.014
复制
发表时间：
2010-03-01
影响因子：
4.1
通讯作者：
Zhu, C. -D.
中科院分区：
文献类型：
--
作者：
Zhang, A. B.;He, L. J.;Zhu, C. -D.
自DNA条形码倡议和全球生物多样性调查开始以来，样本量一直是基本问题之一。作为解决这个问题的贡献，我们提出了一个简单的restaurant的方法来估计几个关键的DNA条形码项目的采样大小。我们说明了我们的方法，使用结构化的人口结合和真实的物种的船长蝴蝶下模拟。我们发现，DNA条形码中广泛使用的样本量不足以评估物种的遗传多样性，种群结构影响样本量的估计，因此可能会导致物种识别的偏差。(C)2009 Elsevier Inc.所有战斗保留。
Sample size has long been one of the basic issues since the start of the DNA barcoding initiative and the global biodiversity investigation. As a contribution to resolving this problem, we propose a simple resampling approach to estimate several key sampling sizes for a DNA barcoding project. We illustrate our approach using both structured Populations simulated under coalescent and real species of skipper butterflies. We found that sample sizes widely used in DNA barcoding are insufficient to assess the genetic diversity of a species, population structure impacts the estimation of the sample sizes, and hence will bias the species identification potentially. (C) 2009 Elsevier Inc. All Fights reserved.